Bagheri Seyyed Majid, Abdian-Asl Amir, Moghadam Mahin Taheri, Yadegari Maryam, Mirjalili Aghdas, Zare-Mohazabieh Fatemeh, Momeni Haniyeh
Dep. of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Neurobiomedical Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Dep. of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
J Ayurveda Integr Med. 2017 Jul-Sep;8(3):152-158. doi: 10.1016/j.jaim.2017.02.013. Epub 2017 Jul 6.
Ferula assa foetida commonly consumed as a healthy beverage has been demonstrated to have various biological activities, including antioxidation, anti-obesity and anti-cancer.
Our study aims to investigate the antitumor effect of asafoetida in vivo using mouse mammary carcinoma 4T1 cells.
In the study, female BALB/c mice were divided into two groups (n = 6), which were control (untreated) and other group of mice with breast cancer treated with 100 mg/kg of asafoetida, respectively, by oral gavage. All mice were injected into the mammary fat pad with 4T1 cells (1 × 10 4T1 cells/0.1 ml of phosphate buffer solution). Asafoetida was administered on day 15 after the tumor had developed for 3 weeks. At end of experiment, tumor weight, tumor volume and tumor burden were measured and lung, liver, kidney and tumor were harvested and sections were prepared for histopathological analysis. Lipoxygenase inhibitory and antioxidant activity of asafoetida was also determined.
Our results showed that treatment with asafoetida was effective in decreasing the tumor weight and tumor volume in treated mice. Body weight significantly increased in female BALB/c mice against control. Apart from the antitumor effect, asafoetida decreased lung, liver and kidney metastasis and also increased areas of necrosis in the tumor tissue respectively.
The present study demonstrated that asafoetida has potent antitumor and antimetastasis effects on breast cancer and is a potential source of natural antitumor products.
阿魏作为一种常见的健康饮品，已被证明具有多种生物活性，包括抗氧化、抗肥胖和抗癌作用。
本研究旨在利用小鼠乳腺癌4T1细胞在体内研究阿魏的抗肿瘤作用。
在本研究中，将雌性BALB/c小鼠分为两组（n = 6），分别为对照组（未治疗）和另一组经口灌胃给予100 mg/kg阿魏的乳腺癌小鼠组。所有小鼠均通过乳腺脂肪垫注射4T1细胞（1×10⁴ 4T1细胞/0.1 ml磷酸盐缓冲溶液）。在肿瘤形成3周后第15天给予阿魏。实验结束时，测量肿瘤重量、肿瘤体积和肿瘤负荷，并采集肺、肝、肾和肿瘤组织，制备切片进行组织病理学分析。同时还测定了阿魏的脂氧合酶抑制活性和抗氧化活性。
我们的结果表明，阿魏治疗可有效降低治疗组小鼠的肿瘤重量和肿瘤体积。与对照组相比，雌性BALB/c小鼠体重显著增加。除抗肿瘤作用外，阿魏还分别减少了肺、肝和肾转移，并增加了肿瘤组织中的坏死面积。
本研究表明，阿魏对乳腺癌具有强大的抗肿瘤和抗转移作用，是天然抗肿瘤产品的潜在来源。
