Bafghi Ali Fatahi, Bagheri Seyyed Majid, Hejazian Seyed Hassan
Department of Parasitoloy, Shahid Sadoghi University of Medical Sciences, Yazd, Iran.
Department of Physiology, Shahid Sadoghi University of Medical Sciences, Yazd, Iran.
J Ayurveda Integr Med. 2014 Oct-Dec;5(4):223-6. doi: 10.4103/0975-9476.146567.
In Ayurveda, asafetida is introduced as a valuable remedy for flatulence, hysteria, nervous disorders, whooping cough, pneumonia and bronchitis in children and also considered as an aphrodisiac agent. Presently, Leishmaniasis is common in most countries of the world and is a serious health problem in the world. Some plant medicines and natural products have a new candidate for treatment of leishmaniasis.
This study was designed to evaluate Ferula assa-foetida oleo gum resin (asafetida) on mortality and morbidity Leishmania major in vitro.
Mostigotes were isolated from mice spleens and then transformed to promastigotes in Novy-Nicolle-Mac Neal (NNN medium supplemented with penicillin (100 U/ml), streptomycin (100 μg/ml) and 20% heat-inactivated fetal calf serum (FCS) at 25°C. A fixed initial density of the parasites was transferred to screw-capped vials containing 5 ml of RPMI1640 media to which different concentrations of 2.5, 5, 10 and 20 μg asafetida were added and each concentration was done in triplicates. Each run also included control. The mortality of parasitoids was measured by the slide and the enzyme-linked immunosorbent assay (ELISA) methods.
After 72 h, asafetida inhibited growth of parasites in all doses in stationary and logarithmic phases. The ELISA measurement suggested that the viability of parasites significantly decreased after 48h (P < 0.05).
The results show that asafetida could prevent from growth and viability of parasites and this oleo gum resin can be useful for treatment of leishmaniasis.
在阿育吠陀医学中，阿魏被视为治疗肠胃胀气、癔症、神经紊乱、百日咳、肺炎以及儿童支气管炎的珍贵药物，还被认为是一种壮阳剂。目前，利什曼病在世界上大多数国家都很常见，是一个严重的健康问题。一些植物药和天然产物成为治疗利什曼病的新候选药物。
本研究旨在评估阿魏油胶树脂（阿魏）对杜氏利什曼原虫体外死亡率和发病率的影响。
从小鼠脾脏中分离出前鞭毛体，然后在添加了青霉素（100 U/ml）、链霉素（100 μg/ml）和20%热灭活胎牛血清（FCS）的诺维 - 尼科尔 - 麦克尼尔（NNN）培养基中，于25°C下将其转化为无鞭毛体。将固定初始密度的寄生虫转移至装有5 ml RPMI1640培养基的螺口小瓶中，向其中添加不同浓度的2.5、5、10和20 μg阿魏，每个浓度设置三个重复。每次实验均设置对照。通过玻片法和酶联免疫吸附测定（ELISA）法测量寄生虫的死亡率。
72小时后，阿魏在所有剂量下均抑制了稳定期和对数期寄生虫的生长。ELISA测量结果表明，48小时后寄生虫的活力显著下降（P < 0.05）。
结果表明，阿魏可抑制寄生虫的生长和活力，这种油胶树脂可用于治疗利什曼病。
