Bakr Mahmoud M, Al-Ankily Mahmoud M, Shogaa Sara M, Shamel Mohamed
School of Medicine and Dentistry, Griffith University, Gold Coast, QLD 4215, Australia.
Faculty of Dentistry, The British University in Egypt, Cairo 11837, Egypt.
Bioengineering (Basel). 2021 Dec 16;8(12):219. doi: 10.3390/bioengineering8120219.
Silver nanoparticles (AgNPs) are extensively used in many industries due to their superior antimicrobial properties. However, it is evident from many studies that AgNPs has cytotoxic potential through its effect on excessive formation of reactive oxygen species (ROS). The aim of this study was to examine the toxic effect of AgNPs on the submandibular salivary glands and the attenuating effect of vitamin E, as a natural antioxidant, against this toxicity. Thirty Albino rats were divided into 3 groups ( = 10): control group, AgNPs group receiving 2 mg/kg daily for 28 days, and AgNPs and vitamin E group receiving AgNPs the same as the previous group in addition to vitamin E at a dose of 100 mg/kg. Microscopic, ultrastructural, and cytokeratin immune-reactivity examination of the glands were performed. The AgNPs group showed noticeable degeneration in all structures of the gland as evident in the histological and ultrastructural examination. The AgNPs and vitamin E group revealed an improvement of the glandular elements. A significant increase in cytokeratin immune expression was found after comparison of both groups ( = 0.01). This current study shows that vitamin E has powerful antioxidant properties, which can combat the cytotoxic effect caused by AgNPs. Further studies are deemed necessary to confirm this finding using other immunohistochemical markers, such as myosin and E-cadherin.
银纳米颗粒（AgNPs）因其卓越的抗菌性能而被广泛应用于许多行业。然而，许多研究表明，AgNPs通过影响活性氧（ROS）的过度形成而具有细胞毒性潜力。本研究的目的是探讨AgNPs对大鼠下颌下唾液腺的毒性作用，以及天然抗氧化剂维生素E对这种毒性的减轻作用。将30只白化大鼠分为3组（每组n = 10）：对照组、连续28天每天接受2 mg/kg AgNPs的AgNPs组、以及除接受与前一组相同剂量的AgNPs外还接受100 mg/kg维生素E的AgNPs与维生素E组。对腺体进行了显微镜、超微结构和细胞角蛋白免疫反应性检查。组织学和超微结构检查显示，AgNPs组腺体的所有结构均出现明显退变。AgNPs与维生素E组的腺细胞成分有所改善。两组比较后发现细胞角蛋白免疫表达显著增加（P = 0.01）。本研究表明，维生素E具有强大的抗氧化特性，可对抗AgNPs引起的细胞毒性作用。有必要进一步开展研究，使用其他免疫组化标记物（如肌球蛋白和E-钙黏蛋白）来证实这一发现。
