Vijayakumar Sujithra, Raj Rajani, Shaanker Uma, Sivaramakrishna Akella, Ramamoorthy Siva
School of Bio Sciences and Technology, VIT, Vellore, Tamil Nadu 632014 India.
Department of Crop Physiology, University of Agricultural Science, Bangalore, Karnataka 560065 India.
3 Biotech. 2021 Jan;11(1):33. doi: 10.1007/s13205-020-02566-x. Epub 2021 Jan 4.
There is a colossal demand for natural pigments and its applications in recent times. In the study, a novel lactone pigment was isolated from a predominant endophytic fungus residing in L. (Bixaceae) leaves. The endophyte was identified as through morphological and molecular investigations. The optimum growth parameters of the endophyte for pigment production were at 33 ºC with pH 6.5 in dark. Through comprehensive spectroscopic studies, the structure of the isolated lactone was resolved and identified as ()-3, 3-dimethyl-4-(pent-1-en-1-yl)-4-propyldihydrofuran-2(3)-one. The acute oral toxicity study of the pigment investigated upon female Wistar rats indicated the median lethal dose (LD) value above 1000 mg/kg body weight affirming safety. Thus, the red pigment from the isolated endophyte may be employed as a sustainable source for natural colorant in industries owing to its non-toxicity.
The online version contains supplementary material available at 10.1007/s13205-020-02566-x.
近年来，对天然色素及其应用有着巨大的需求。在本研究中，从一种存在于红木科（Bixaceae）植物叶片中的优势内生真菌中分离出一种新型内酯色素。通过形态学和分子学研究确定了该内生真菌。该内生真菌产生色素的最佳生长参数为33℃、pH值6.5且处于黑暗环境。通过全面的光谱研究，解析并鉴定出分离出的内酯结构为( )-3, 3-二甲基-4-(戊-1-烯-1-基)-4-丙基二氢呋喃-2(3)-酮。对雌性Wistar大鼠进行的该色素急性经口毒性研究表明，其半数致死剂量（LD）值高于1000 mg/kg体重，证实了其安全性。因此，由于其无毒，从分离出的内生真菌中获得的红色素可作为工业中天然色素的可持续来源。
在线版本包含可在10.1007/s13205-020-02566-x获取的补充材料。
