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濒危中国物种叶绿体全基因组的特征分析

Characterization of the complete chloroplast genome of the endangered Chinese species .

作者信息

Cheng Longjie, Wang Yuying, Zhao Yiran, Li Yefang, He Fengmei, Li Zhilin

机构信息

College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.

出版信息

Mitochondrial DNA B Resour. 2020 Sep 4;5(3):3318-3319. doi: 10.1080/23802359.2020.1815603.

DOI:10.1080/23802359.2020.1815603
PMID:33458150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7782137/
Abstract

is a Class I endangered species in China with important ornamental, economic, and breeding value, especially wild This study used Illumina high-throughput sequencing technologies to sequence and analyze the complete chloroplast genome of . The genome features of and the phylogenetic relationships among Orchidaceae species were reported and established. The complete chloroplast genome is 155,447 bp long, consisting of a pair of inverse duplication regions that are 26,710 bp long, a large single-copy region of 84,184 bp, and a small single-copy region of 17,843 bp. The entire genome contains 74 mRNA genes, 30 tRNA genes, and 4 rRNA genes. The phylogenetic tree of 24 Orchidaceae species revealed is more closely related to .

摘要

是中国国家一级保护植物,具有重要的观赏、经济和育种价值,尤其是野生的。本研究采用Illumina高通量测序技术对其完整叶绿体基因组进行测序和分析。报道并建立了其基因组特征以及兰科物种之间的系统发育关系。完整的叶绿体基因组长度为155,447 bp,由一对长度为26,710 bp的反向重复区域、一个长度为84,184 bp的大单拷贝区域和一个长度为17,843 bp的小单拷贝区域组成。整个基因组包含74个mRNA基因、30个tRNA基因和4个rRNA基因。24种兰科植物的系统发育树显示,与关系更密切。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1598/7782137/e2262b29b638/TMDN_A_1815603_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1598/7782137/e2262b29b638/TMDN_A_1815603_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1598/7782137/e2262b29b638/TMDN_A_1815603_F0001_B.jpg

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本文引用的文献

1
GeSeq - versatile and accurate annotation of organelle genomes.GeSeq - 细胞器基因组的多功能和准确注释。
Nucleic Acids Res. 2017 Jul 3;45(W1):W6-W11. doi: 10.1093/nar/gkx391.
2
NOVOPlasty: de novo assembly of organelle genomes from whole genome data.NOVOPlasty:从头组装细胞器基因组的全基因组数据。
Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
3
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.MEGA7:适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.