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一种中国濒危物种叶绿体全基因组的特征分析

Characterization of the complete chloroplast genome of a Chinese endangered species .

作者信息

Wang Yuying, Cheng Longjie, Zhao Yiran, Li Yefang, He Fengmei, Li Zhilin

机构信息

College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.

出版信息

Mitochondrial DNA B Resour. 2020 Aug 25;5(3):3199-3200. doi: 10.1080/23802359.2020.1810159.

DOI:10.1080/23802359.2020.1810159
PMID:33458111
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7782536/
Abstract

is an endangered species belonging to the first ranking in protection category in China with important ornamental value and breeding value. This study used Illumina high-throughput sequencing technologies to sequence and analyze the complete chloroplast genome of The genome features of and the phylogenetic relationships among Orchidaceae species were reported and established. The complete chloroplast genome is 152,544 bp in length, consisting of a pair of inverse duplication regions 25,357 bp, a large single-copy region 86,014 bp and a small single-copy region 15,816 bp. The entire genome contains 74 mRNA genes, 30 tRNA genes and 4 rRNA genes. The phylogenetic tree of 23 Orchidaceae species revealed is more closely related to .

摘要

是中国国家重点一级保护野生植物,具有重要的观赏价值和科研价值。本研究利用Illumina高通量测序技术对其叶绿体全基因组进行测序分析,报道并构建了该植物的基因组特征及与兰科其他物种间的系统发育关系。其叶绿体全基因组长度为152,544 bp,包括一对25,357 bp的反向重复序列、一个86,014 bp的大单拷贝区和一个15,816 bp的小单拷贝区。全基因组共包含74个mRNA基因、30个tRNA基因和4个rRNA基因。23种兰科植物的系统发育树显示,该植物与 关系更近。

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本文引用的文献

1
GeSeq - versatile and accurate annotation of organelle genomes.GeSeq - 细胞器基因组的多功能和准确注释。
Nucleic Acids Res. 2017 Jul 3;45(W1):W6-W11. doi: 10.1093/nar/gkx391.
2
NOVOPlasty: de novo assembly of organelle genomes from whole genome data.NOVOPlasty:从头组装细胞器基因组的全基因组数据。
Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
3
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.MEGA7:适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.