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一种中国濒危物种叶绿体全基因组的特征分析

Characterization of the complete chloroplast genome of a Chinese Endangered species .

作者信息

Ling Qing, Cheng Longjie, Li Zhilin, Zhao Yiran, Wang Yuying

机构信息

College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.

Yunnan Vocational and Technical College of Agriculture, Kunming, China.

出版信息

Mitochondrial DNA B Resour. 2021 Mar 15;6(3):708-709. doi: 10.1080/23802359.2020.1851152.

DOI:10.1080/23802359.2020.1851152
PMID:33796643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7971198/
Abstract

is an endangered species belonging to the first ranking in protection category in China with important ornamental value and breeding value. This study used Illumina high-throughput sequencing technologies for of chloroplast genome sequencing. The genome features of and the phylogenetic relationships were reported and established. The complete chloroplast genome is 156,317 bp in length, consisting of a pair of inverse duplication regions 26,544 bp, a large single-copy region 85,360 bp and a small single-copy region 17,869 bp. The entire genome contains 73 mRNA genes, 30 tRNA genes and 4 rRNA genes. The phylogenetic tree of 23 Orchidaceae species revealed is more closely related to .

摘要

是中国国家重点一级保护野生植物,具有重要的观赏价值和科研价值。本研究采用Illumina高通量测序技术对其叶绿体基因组进行测序,报道并构建了其基因组特征及系统发育关系。其完整叶绿体基因组长度为156,317 bp,包括一对26,544 bp的反向重复序列、一个85,360 bp的大单拷贝区和一个17,869 bp的小单拷贝区。全基因组共包含73个mRNA基因、30个tRNA基因和4个rRNA基因。23种兰科植物的系统发育树显示,与关系更近。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af95/7971198/0d2d3bdd6cf0/TMDN_A_1851152_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af95/7971198/0d2d3bdd6cf0/TMDN_A_1851152_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af95/7971198/0d2d3bdd6cf0/TMDN_A_1851152_F0001_B.jpg

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本文引用的文献

1
The complete chloroplast genome of an endangered species (Orchidaceae).一种濒危物种(兰科)的完整叶绿体基因组。
Mitochondrial DNA B Resour. 2019 Sep 19;4(2):3068-3069. doi: 10.1080/23802359.2019.1666047.
2
GeSeq - versatile and accurate annotation of organelle genomes.GeSeq - 细胞器基因组的多功能和准确注释。
Nucleic Acids Res. 2017 Jul 3;45(W1):W6-W11. doi: 10.1093/nar/gkx391.
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NOVOPlasty: de novo assembly of organelle genomes from whole genome data.NOVOPlasty:从头组装细胞器基因组的全基因组数据。
Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
4
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.MEGA7:适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.