尿液中的人生长激素：一种超灵敏放射免疫测定法的开发

Human growth hormone in urine: development of an ultrasensitive radiometric assay.

作者信息

Erb T, Karolyi G, Pampalone A, Eberle A N, Baumann J B, Bürgisser E, Girard J

机构信息

University Children's Hospital, Basel, Switzerland.

出版信息

Experientia. 1988 Feb 15;44(2):155-7. doi: 10.1007/BF01952201.

DOI:10.1007/BF01952201

PMID:3345820

Abstract

An immunoradiometric assay for human growth hormone (HGH) has been developed which has a detection limit of 1 ng/l and can measure HGH in unextracted urine from normal children and adults. The assay is based on a two-step procedure, using a solid-phase goat-anti-HGH immunosorbent for immunoextraction and [125I]-labeled monoclonal HGH-antibody for detection and quantification. The assay is not affected by urea, NaCl or changes of pH from 5-8. The mean urine HGH concentration in normal children is 6.78 +/- 7.6 (SD) pg/ml, in patients with HGH-deficiency 1.3 +/- 0.9 pg/ml which increases to 11.7 +/- 13.4 pg/ml on the day of growth hormone injection.

摘要

已开发出一种用于检测人生长激素（HGH）的免疫放射分析方法，其检测限为1 ng/l，可对正常儿童和成人未提取的尿液中的HGH进行检测。该分析方法基于两步程序，使用固相山羊抗HGH免疫吸附剂进行免疫提取，并使用[125I]标记的单克隆HGH抗体进行检测和定量。该分析不受尿素、氯化钠或pH值在5至8之间变化的影响。正常儿童尿液中HGH的平均浓度为6.78 +/- 7.6（标准差）pg/ml，生长激素缺乏症患者为1.3 +/- 0.9 pg/ml，在注射生长激素当天增加至11.7 +/- 13.4 pg/ml。