在自由活动的小鼠中对新生神经元进行钙成像。
Calcium imaging of adult-born neurons in freely moving mice.
机构信息
Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), Tokyo 113-0033, Japan.
出版信息
STAR Protoc. 2020 Dec 31;2(1):100238. doi: 10.1016/j.xpro.2020.100238. eCollection 2021 Mar 19.
Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).
成体神经发生（ABNs）在齿状回赋予哺乳动物大脑独特的细胞可塑性。我们最近发现，睡眠期间 ABN 的活动对于记忆巩固是必要的。在这里，我们描述了使用微型荧光显微镜和睡眠记录进行 ABN 活性钙成像的方法。由于预备手术和记录后的数据处理可能是主要障碍，我们提供了详细的描述和问题解决技巧。有关此协议的使用和执行的完整详细信息，请参阅 Kumar 等人。（2020 年）。
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