从小鼠中制备和培养骨髓来源的巨噬细胞用于功能分析。
Preparation and culture of bone marrow-derived macrophages from mice for functional analysis.
机构信息
Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.
Toranomon Hospital, Tokyo, Japan.
出版信息
STAR Protoc. 2020 Dec 31;2(1):100246. doi: 10.1016/j.xpro.2020.100246. eCollection 2021 Mar 19.
The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).
由于存在多种亚型，巨噬细胞功能的评估一直是一个热门话题。本方案描述了从小鼠股骨和胫骨中收集骨髓细胞、将其分化为骨髓来源的巨噬细胞（BMDM 细胞）以及从培养物中取样的过程。本方案侧重于高效制备 BMDM 细胞，为评估巨噬细胞的功能提供了一种方法。如需详细了解本方案的使用和执行，请参考 Toda 等人（2020 年）的文献。
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