Feng Lingyu, Li Jiang, Sun Jielin, Wang Lihua, Fan Chunhai, Shen Jianlei
Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Adv Healthc Mater. 2021 Mar;10(6):e2001718. doi: 10.1002/adhm.202001718. Epub 2021 Jan 18.
Materials that can regulate the composition and structure of the cell membrane to fabricate engineered cells with defined functions are in high demand. Compared with other biomolecules, DNA has unique advantages in cell membrane engineering due to its excellent programmability and biocompatibility. Especially, the near-atomic scale precision of DNA nanostructures facilitates the investigation of structure-property relations on the cell membrane. In this review, first the state of the art of functional DNA nanostructures is summarized, and then the overview of the use of DNA nanostructures to engineer the cell membrane is presented. Subsequently, applications of DNA nanostructures in modifying cell membrane morphology, controlling ions transport, and synthesizing high precise liposomes are highlighted. Finally, the challenges and outlook on using DNA nanostructures for cell membrane engineering are discussed.
能够调节细胞膜的组成和结构以制造具有特定功能的工程细胞的材料需求旺盛。与其他生物分子相比，DNA因其出色的可编程性和生物相容性，在细胞膜工程中具有独特优势。特别是，DNA纳米结构的近原子尺度精度有助于研究细胞膜上的结构-性质关系。在本综述中，首先总结了功能性DNA纳米结构的研究现状，然后介绍了利用DNA纳米结构对细胞膜进行工程改造的概况。随后，重点介绍了DNA纳米结构在修饰细胞膜形态、控制离子运输和合成高精度脂质体方面的应用。最后，讨论了使用DNA纳米结构进行细胞膜工程面临的挑战和前景。
