Lissi E A, Cáceres T, Videla L A
J Free Radic Biol Med. 1986;2(1):63-9. doi: 10.1016/0748-5514(86)90125-x.
Rat brain homogenate autoxidation was assessed from thiobarbituric acid reactant accumulation (TBAR), light emission, and oxygen uptake. The effect of several additives upon TBAR accumulation and light intensity suggests that these parameters can be employed as a reliable measure of the lipoperoxidation extent. From the different time profiles of TBAR accumulation and light emission, it is concluded that instantaneous light emission is not a measure of the lipoperoxidation rate but it is related to the accumulation of products. The time dependence of the light emitted after addition to an incubated sample of an excess of free radical scavengers indicates that at least two intermediates of widely different lifetimes are contributing to the observed light emission.
通过硫代巴比妥酸反应物积累（TBAR）、发光和氧摄取来评估大鼠脑匀浆的自氧化。几种添加剂对TBAR积累和光强度的影响表明，这些参数可用作脂质过氧化程度的可靠指标。从TBAR积累和发光的不同时间曲线可以得出结论，瞬时发光不是脂质过氧化速率的指标，但它与产物的积累有关。向孵育样品中添加过量自由基清除剂后发光的时间依赖性表明，至少有两种寿命差异很大的中间体对观察到的发光有贡献。
