Penhaul Smith J K, Hughes A D, McEvoy L, Thornton B, Day J G
Scottish Association for Marine Science, Argyll and Bute, Oban, PA37 1QA, Scotland, UK.
NAFC Marine Centre, Port Arthur, Scalloway, ZE1 0UN, Scotland, UK.
Biotechnol Lett. 2021 Mar;43(3):729-743. doi: 10.1007/s10529-020-03073-y. Epub 2021 Jan 18.
Changes in the partitioning of dissolved inorganic (DIC) and glucose were elucidated by utilising C labelled DIC or glucose, and quantifying the biochemical profile of mixotrophic, heterotrophic and photoautotrophic cultures of the microalga Tetraselmis suecica.
Mixotrophic cultivation increases microalgal productivity and changes their biochemical profile, due to an alteration in the partitioning of carbon within the cell. When cultured mixotrophically and heterotrophically, there is enhanced incorporation of carbon into shorter chain saturated fatty acids and non-lipid biomass, compared to photoautotrophic cultivation. Autotrophic culture results in increased total fatty acid content of cultures (4.19% dry weight compared to 2.13%) and shifts the fatty acid profile in favour of long-chain unsaturated fatty acids, such as 18:2 n-(9,12), compared to mixotrophic culture. Quantifying the changes in partitioning between DIC and glucose facilitates tailoring of the biochemical profile to develop "designer" algae.
There is a condition specific shift in carbon partitioning into different fatty acid and biochemical fractions in T. suecica, with more inorganic carbon partitioned into 18:2 n-(9,12) in photoautotrophic rather than mixotrophic cultures.
通过利用碳标记的溶解无机碳（DIC）或葡萄糖，并对微藻瑞典四爿藻的混合营养、异养和光合自养培养物的生化特征进行量化，阐明溶解无机碳（DIC）和葡萄糖分配的变化。
混合营养培养提高了微藻的生产力并改变了它们的生化特征，这是由于细胞内碳分配的改变。与光合自养培养相比，当进行混合营养培养和异养培养时，碳向短链饱和脂肪酸和非脂质生物量中的掺入增加。自养培养导致培养物的总脂肪酸含量增加（干重为4.19%，而光合自养培养为2.13%），并且与混合营养培养相比，脂肪酸谱向有利于长链不饱和脂肪酸（如18:2 n-(9,12)）的方向转变。量化DIC和葡萄糖之间分配的变化有助于定制生化特征以培育“设计型”藻类。
在瑞典四爿藻中，碳分配到不同脂肪酸和生化组分存在特定条件下的转变，在光合自养培养而非混合营养培养中，更多的无机碳分配到18:2 n-(9,12)中。
