Medical Microbiology, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; National Health Laboratory Service, South Africa; Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
J Glob Antimicrob Resist. 2021 Mar;24:175-177. doi: 10.1016/j.jgar.2020.12.021. Epub 2021 Jan 15.
The underlying resistance mechanisms, defence systems, mobilome, virulome, clonality and global phylogenetic relationship of a novel sequence type (ST) 658 Aeromonas hydrophilia (A34a) isolated from a pig abattoir in South Africa was determined using whole-genome sequence (WGS) technology.
Following isolation on chromogenic agar (CHROMID® CARBA SMART), microbial identification and antibiotic susceptibility testing were performed using a VITEK®2 platform. Genotyping involved WGS performed with an Illumina MiSeq platform.
The antibiotic resistome agreed with the resistance phenotype of the isolate and included antibiotic resistance determinants for β-lactams (bla and bla). BLASTn analysis of resistome-encoding contigs affirmed chromosomally-mediated resistance. BURST algorithmic analysis identified the novel ST658 as a satellite variant. Virulome analysis predicted virulence genes of Aeromonas whose expression are critical for establishing infection in the host. Global phylogenomic analyses showed strain A34a is closely related to two international isolates from Sri Lanka (Ae25) and the USA (RU34A), although there is little to suggest that it was imported from abroad.
This is the first report on the genomic analysis of a novel ST658 A. hydrophilia, offering useful insights into its pathogenicity and global phylogenetics.
利用全基因组测序（WGS）技术，确定从南非一家养猪场分离的新型 658 型嗜水气单胞菌（A34a）的潜在耐药机制、防御系统、移动元件、毒力组、克隆性和全球系统发育关系。
在显色琼脂（CHROMID® CARBA SMART）上分离后，使用 VITEK®2 平台进行微生物鉴定和抗生素药敏试验。基因分型包括使用 Illumina MiSeq 平台进行的 WGS。
抗生素耐药组与分离物的耐药表型一致，包括β-内酰胺类（bla 和 bla）的抗生素耐药决定因子。耐药组编码基因座的 BLASTn 分析证实了染色体介导的耐药性。BURST 算法分析确定新型 ST658 为卫星变体。毒力组分析预测了气单胞菌的毒力基因，其表达对在宿主中建立感染至关重要。全球系统发育分析表明，菌株 A34a 与来自斯里兰卡（Ae25）和美国（RU34A）的两个国际分离株密切相关，尽管几乎没有证据表明它是从国外进口的。
这是首次对新型 658 型嗜水气单胞菌的基因组分析报告，为其致病性和全球系统发育提供了有用的见解。
