Hirashima S, Hirai H, Nakanishi Y, Natori S
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
J Biol Chem. 1988 Mar 15;263(8):3858-63.
Complementary DNA (cDNA) clones encoding a transcription factor S-II were isolated and characterized. The primary structure of S-II was determined by nucleotide sequence analysis of these clones. The predicted primary structure was consistent with the model that we proposed previously from the results of biochemical analyses of S-II. Using these clones as probes, we analyzed the mRNA for S-II. RNA blot analysis demonstrated the presence of four species of mRNA that hybridized with S-II cDNA in Ehrlich ascites tumor cells. This is the first evidence of polymorphism of mRNA encoding a transcription factor of RNA polymerase II. The results of analysis of the genomic structure suggested that the polymorphism of mRNA may be due to alternative splicing, or differences in initiation or termination of transcription.
编码转录因子S-II的互补DNA（cDNA）克隆被分离并进行了表征。通过对这些克隆的核苷酸序列分析确定了S-II的一级结构。预测的一级结构与我们先前根据S-II的生化分析结果提出的模型一致。使用这些克隆作为探针，我们分析了S-II的mRNA。RNA印迹分析表明，在艾氏腹水瘤细胞中存在四种与S-II cDNA杂交的mRNA。这是编码RNA聚合酶II转录因子的mRNA多态性的首个证据。基因组结构分析结果表明，mRNA的多态性可能是由于可变剪接，或转录起始或终止的差异所致。
