School of Pharmacy, Xuzhou Medical University, 221004 Xuzhou, China.
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002 Xuzhou, China.
ACS Biomater Sci Eng. 2020 Jan 13;6(1):575-586. doi: 10.1021/acsbiomaterials.9b01381. Epub 2019 Dec 13.
Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio. In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried. The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested. The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues. The synthesized composite scaffold has high porosity and a certain mechanical strength. By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction. In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area. All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
三维蜂窝状多孔碳 (HPC) 由于具有优异的机械性能和高的比表面积，在生物工程中受到越来越多的关注。本文通过在 HPC 表面原位沉积纳米级羟基磷灰石 (nHA)、溶解在壳聚糖溶液中并进行真空冷冻干燥的方法，制备了一种三维壳聚糖 (CS)/蜂窝状多孔碳/羟基磷灰石复合材料。采用扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和 X 射线光电子能谱对 CS/HPC/nHA 的结构和组成进行了表征，并对支架的孔隙率、溶胀率和机械性能进行了测试。所制备的支架具有分级孔和有机-无机成分，其组成和结构与骨组织相似。合成的复合支架具有较高的孔隙率和一定的机械强度。通过在支架表面培养小鼠骨髓间充质干细胞，证实了支架有利于其生长，并促进其向成骨方向分化。体内实验进一步证明，CS/HPC/nHA 复合支架在促进骨缺损区域骨形成方面具有显著优势。所有结果表明，CS/HPC/nHA 支架在骨组织工程中有很好的应用前景。
