Chen J D, Yew F H, Li G C
Department of Zoology, National Taiwan University, Taipei, ROC.
J Cell Physiol. 1988 Feb;134(2):189-99. doi: 10.1002/jcp.1041340204.
The growth of tissue culture TO-2 cells derived from the warm water fish Tilapia, the induction of thermotolerance, and protein synthesis profiles of these cells in response to temperature changes were examined. TO-2 cells can grow between 15 to 34 degrees, with an optimal growth temperature of 31 degrees. There is no apparent killing of the cells when the temperature is lowered to 4 degrees for up to 3 days. Survival of TO-2 cells at 43 degrees was studied after various preheat treatments: 1) acute heating at 40 degrees for 15 min followed by 31 degrees incubation, 2) chronic exposure at 37 degrees for several hr, or 3) long-term thermal adaptation at 34 degrees. The cells acquire thermotolerance from pre-exposure to 37 degrees for as short as 6 hr. Preheating at 40 degrees followed by incubation at 31 degrees also induces thermotolerance against a subsequent 43 degrees heat challenge. In addition, 34 degrees thermal adapted cells are resistant to 43 degrees heating. One- and two-dimensional gel electrophoresis of proteins after heat treatments show that three major heat shock proteins with molecular weights around 87, 70, and 27 kD are preferentially synthesized. The synthesis of two additional proteins with an isoelectric point of 6.9 and molecular weights of 60 and 44 kD are significantly enhanced in 34 degrees thermal-adapted and 37 degrees chronic heated cells, but not in cells subjected to an acute heat shock at either 40 degrees or 43 degrees. On the other hand, the 27 kD heat shock proteins are mainly present in the 43 degrees, 40 degrees, and 37 degrees heat-shocked cells, but not in the 34 degrees thermal-adapted cells.
研究了源自温水鱼类罗非鱼的组织培养TO - 2细胞的生长、耐热性诱导以及这些细胞在温度变化时的蛋白质合成概况。TO - 2细胞能在15至34摄氏度之间生长，最佳生长温度为31摄氏度。当温度降至4摄氏度长达3天时，细胞没有明显死亡。在进行各种预热处理后，研究了TO - 2细胞在43摄氏度时的存活率：1）在40摄氏度下急性加热15分钟，然后在31摄氏度下孵育；2）在37摄氏度下慢性暴露数小时；3）在34摄氏度下长期热适应。细胞在短至6小时的37摄氏度预暴露后即可获得耐热性。在40摄氏度预热后于31摄氏度孵育也能诱导对随后43摄氏度热刺激的耐热性。此外，34摄氏度热适应的细胞对43摄氏度加热具有抗性。热处理后蛋白质的一维和二维凝胶电泳显示，优先合成了分子量约为87、70和27 kD的三种主要热休克蛋白。在34摄氏度热适应和37摄氏度慢性加热的细胞中，另外两种等电点为6.9、分子量为60和44 kD的蛋白质的合成显著增强，但在40摄氏度或43摄氏度急性热休克的细胞中则没有。另一方面，27 kD热休克蛋白主要存在于43摄氏度、40摄氏度和37摄氏度热休克的细胞中，而不存在于34摄氏度热适应的细胞中。
