Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004623.
A Gram-stain-negative, aerobic, short rod-shaped, pale yellow-pigmented, non-motile and gentamycin-resistant bacterial strain designated CJ210 was isolated from the Han River, Republic of Korea. Strain CJ210 grew optimally at 30 °C and pH 7.0 in the absence of NaCl on tryptic soy agar. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain CJ210 belonged to the genus within the family and was most closely related to KACC 14347 (98.1 % similarity), followed by KCTC 23367 (95.3 % similarity). The average nucleotide identity values between strain CJ210 and two closely related type strains KACC 14347 and KCTC 23367 were 83.7 and 73.8 %, respectively. The digital DNA-DNA hybridization results between strain CJ210 and the related type strains were 27.5 and 20.2 %, respectively. Strain CJ210 contained menaquinone 6 (MK-6) as the predominant menaquinone. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The major fatty acids of strain CJ210 were iso-C, iso-C 3-OH and summed feature 9 (comprising iso-C 9 and/or C 10-methyl). Whole genome sequencing revealed that strain CJ210 had a genome of 3.8 Mbp with 36.5 % DNA G+C content. The genome contained several antimicrobial resistance genes including an aminoglycoside-resistant gene. On the basis of the polyphasic taxonomic study, strain CJ210 represents a novel species in the genus , for which name sp. nov. is proposed. The type strain is CJ210 (=KACC 19954=JCM 33306).
一株革兰氏阴性、需氧、短杆状、浅黄色素、非运动和庆大霉素抗性的细菌菌株 CJ210 从韩国汉江中分离得到。菌株 CJ210 在无 NaCl 的胰蛋白胨大豆琼脂上最适生长温度为 30°C，pH 值为 7.0。不产生 Flexirubin 型色素。基于 16S rRNA 基因序列相似性的系统发育分析表明，菌株 CJ210 属于 科中的 属，与 KACC 14347 最为密切相关（98.1%相似性），其次是 KCTC 23367（95.3%相似性）。菌株 CJ210 与两个密切相关的模式菌株 KACC 14347 和 KCTC 23367 的平均核苷酸同一性值分别为 83.7%和 73.8%。菌株 CJ210 与相关模式株的数字 DNA-DNA 杂交值分别为 27.5%和 20.2%。菌株 CJ210 含有menaquinone 6 (MK-6) 作为主要的menaquinone。主要的极性脂为磷脂酰乙醇胺、两种未鉴定的胺脂和两种未鉴定的脂。菌株 CJ210 的主要脂肪酸为 iso-C、iso-C 3-OH 和总和特征 9（包含 iso-C 9 和/或 C 10-甲基）。全基因组测序表明，菌株 CJ210 的基因组大小为 3.8 Mbp，GC 含量为 36.5%。基因组包含几个抗生素抗性基因，包括氨基糖苷类抗性基因。基于多相分类学研究，菌株 CJ210 代表了 属中的一个新种，建议命名为 sp. nov.。模式菌株为 CJ210（=KACC 19954=JCM 33306）。
