National Public Health Laboratory, National Centre for Infectious Diseases, Block G, Level 13, 16 Jalan Tan Tock Seng, Singapore 308442, Singapore.
Parasite. 2021;28:6. doi: 10.1051/parasite/2021002. Epub 2021 Jan 19.
Dermacentor (Indocentor) auratus Supino, 1897 is a prominent ixodid vector of numerous pathogens of public health and veterinary importance. Using long-range PCR of two overlapping regions sequenced on an Illumina MiSeq machine, the complete mitochondrial genome of D. auratus is reported here. The resulting contigs were able to be assembled into a complete and circularised genome which had the general organisation of the mitochondrial genomes of the Metastriates. It had a total length of 14,766 bp and contained 37 genes, including 13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes, as well as 2 non-coding control regions and 3 tick-boxes. The phylogenetic analysis on the whole mitogenome confirmed the position of D. auratus within the Dermacentor clade.
金盾革蜱(Indocentor)auratus Supino,1897 是一个重要的革螨,携带许多对公共卫生和兽医具有重要意义的病原体。本研究使用 Illumina MiSeq 测序仪对两个重叠区域进行长程 PCR,报道了金盾革蜱的完整线粒体基因组。所得的重叠片段能够组装成一个完整的环状基因组,其组织结构与 Metastriates 的线粒体基因组相似。该基因组全长 14766bp,包含 37 个基因,包括 13 个蛋白编码基因、22 个转移 RNA 基因和 2 个核糖体 RNA 基因,以及 2 个非编码控制区和 3 个蜱盒。基于整个线粒体基因组的系统发育分析证实了金盾革蜱在 Dermacentor 进化枝中的位置。