Department of Oncology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China.
Department of Hemodialysis, Nanchang First Hospital, Nanchang, Jiangxi, People's Republic of China.
Hum Cell. 2021 Mar;34(2):478-490. doi: 10.1007/s13577-020-00478-9. Epub 2021 Jan 19.
It has been corroborated that long noncoding RNA (lncRNA) played fundamental function in various human malignancies development including lung adenocarcinoma (lung ADC). In our study, LINC00520 roles in lung ADC tumorigenesis were explored. We found that LINC00520 level was elevated in lung ADC tissues and cell lines. Besides, the LINC00520 expression had a negative connection with miR-1252-5p level in lung ADC tissues. Additionally, our results demonstrated the reciprocal repression influence between LINC00520 and miR-1252-5p. Moreover, luciferase reporter assays, RIP (RNA-binding protein immunoprecipitation) and pull down assays revealed that miR-1252-5p regulated LINC00520 in RISC-dependent. Furthermore, knockdown of LINC00520 inhibited lung ADC cells proliferation, migration and invasion, while co-transfection with a miR-1252-5p inhibitor inverted these influences. Additionally, the findings also demonstrated that FOXR2 was a target of miR-1252-5p; thus, LINC00520 could regulate FOXR2 level. Moreover, LINC00520 silencing suppressed the tumor growth of lung ADC in vivo. In summary, our data indicated that LINC00520 may act as a ceRNA to modulated FOXR2 level by sponging miR-1252-5p, which might bring a potential and effective biomarker to lung ADC treatment.
已证实长链非编码 RNA（lncRNA）在包括肺腺癌（lung ADC）在内的多种人类恶性肿瘤的发展中发挥着重要作用。在我们的研究中，探讨了 LINC00520 在 lung ADC 肿瘤发生中的作用。我们发现 LINC00520 水平在 lung ADC 组织和细胞系中升高。此外，LINC00520 的表达与 lung ADC 组织中的 miR-1252-5p 水平呈负相关。此外，我们的结果表明 LINC00520 和 miR-1252-5p 之间存在相互抑制的影响。此外，荧光素酶报告基因检测、RIP（RNA 结合蛋白免疫沉淀）和下拉实验表明，miR-1252-5p 通过 RISC 依赖性调控 LINC00520。此外，敲低 LINC00520 抑制 lung ADC 细胞的增殖、迁移和侵袭，而共转染 miR-1252-5p 抑制剂则反转了这些影响。此外，研究结果还表明 FOXR2 是 miR-1252-5p 的靶标；因此，LINC00520 可以调节 FOXR2 水平。此外，LINC00520 沉默抑制了 lung ADC 的体内肿瘤生长。总之，我们的数据表明 LINC00520 可能通过海绵吸附 miR-1252-5p 作为 ceRNA 来调节 FOXR2 水平，这可能为 lung ADC 治疗带来潜在的有效生物标志物。
