Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States of America.
Department of Medicine, Division of Infectious Diseases, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States of America.
PLoS One. 2021 Jan 19;16(1):e0245219. doi: 10.1371/journal.pone.0245219. eCollection 2021.
Hepatic steatosis (HS) is common in adolescents with obesity and polycystic ovary syndrome (PCOS). Gut microbiota are altered in adults with obesity, HS, and PCOS, which may worsen metabolic outcomes, but similar data is lacking in youth.
Thirty-four adolescents with PCOS and obesity underwent stool and fasting blood collection, oral glucose tolerance testing, and MRI for hepatic fat fraction (HFF). Fecal bacteria were profiled by high-throughput 16S rRNA gene sequencing.
50% had HS (N = 17, age 16.2±1.5 years, BMI 38±7 kg/m2, HFF 9.8[6.5, 20.7]%) and 50% did not (N = 17, age 15.8±2.2 years, BMI 35±4 kg/m2, HFF 3.8[2.6, 4.4]%). The groups showed no difference in bacterial α-diversity (richness p = 0.202; evenness p = 0.087; and diversity p = 0.069) or global difference in microbiota (β-diversity). Those with HS had lower % relative abundance (%RA) of Bacteroidetes (p = 0.013), Bacteroidaceae (p = 0.009), Porphyromonadaceae (p = 0.011), and Ruminococcaceae (p = 0.008), and higher Firmicutes:Bacteroidetes (F:B) ratio (47.8% vs. 4.3%, p = 0.018) and Streptococcaceae (p = 0.034). Bacterial taxa including phyla F:B ratio, Bacteroidetes, and family Bacteroidaceae, Ruminococcaceae and Porphyromonadaceae correlated with metabolic markers.
Obese adolescents with PCOS and HS have differences in composition of gut microbiota, which correlate with metabolic markers, suggesting a modifying role of gut microbiota in HS and PCOS.
肝脂肪变性（HS）在肥胖和多囊卵巢综合征（PCOS）青少年中很常见。肥胖、HS 和 PCOS 成人的肠道微生物群发生改变，这可能会使代谢结果恶化，但在年轻人中缺乏类似数据。
34 名患有 PCOS 和肥胖的青少年接受粪便和空腹血液采集、口服葡萄糖耐量测试和肝脏脂肪分数（HFF）的 MRI。通过高通量 16S rRNA 基因测序对粪便细菌进行分析。
50%（N=17）的患者存在 HS（年龄 16.2±1.5 岁，BMI 38±7kg/m2，HFF 9.8[6.5，20.7]%），50%（N=17）的患者不存在 HS（年龄 15.8±2.2 岁，BMI 35±4kg/m2，HFF 3.8[2.6，4.4]%）。两组间细菌 α-多样性（丰富度 p=0.202；均匀度 p=0.087；多样性 p=0.069）或微生物群全局差异（β-多样性）无差异。HS 患者的拟杆菌门（Bacteroidetes）（p=0.013）、拟杆菌科（Bacteroidaceae）（p=0.009）、卟啉单胞菌科（Porphyromonadaceae）（p=0.011）和真杆菌科（Ruminococcaceae）（p=0.008）的相对丰度（%RA）较低，而厚壁菌门/拟杆菌门（Firmicutes/Bacteroidetes）（F:B）比值（47.8% vs. 4.3%，p=0.018）和链球菌科（Streptococcaceae）（p=0.034）较高。包括 F:B 比值、拟杆菌门、拟杆菌科、真杆菌科和卟啉单胞菌科在内的细菌分类群与代谢标志物相关。
患有 PCOS 和 HS 的肥胖青少年肠道微生物群组成存在差异，与代谢标志物相关，提示肠道微生物群在 HS 和 PCOS 中起调节作用。
