Diabetes Obesity and Human Reproduction Research Group, Department of Endocrinology and Nutrition, Hospital Universitario Ramón y Cajal & Universidad de Alcalá, Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
Department of Microbiology, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
J Clin Endocrinol Metab. 2018 Jul 1;103(7):2552-2562. doi: 10.1210/jc.2017-02799.
Gut microbiota play a major role in health and disease by influencing physiology, metabolism, nutrition, and immune function.
To evaluate the composition of gut microbiota in women with polycystic ovary syndrome (PCOS), focusing on the influence of sex, sex hormones and obesity on the associations found.
Cross-sectional study.
Academic hospital.
We recruited 15 women with PCOS, 16 nonhyperandrogenic control women, and 15 control men. Participants were classified as nonobese (<30 kg/m2) or obese (≥30 kg/m2) according to their body mass index.
Standardization of diet for 3 consecutive days (at least 300 g of carbohydrates per day) followed by fecal sampling and a standard 75-g oral glucose tolerance test.
Analysis of bacterial abundance and composition of gut microbiota by massive sequencing of 16S ribosomal DNA amplicons in a MiSeq platform (Illumina).
α Bacterial diversity was reduced in women compared with men, and β diversity was reduced particularly in obese patients with PCOS. Women with PCOS presented with specific abnormalities in gut microbiota consisting of an increased abundance of the Catenibacterium and Kandleria genera. When all participants as a whole were considered, indexes of bacterial diversity and the abundance of several bacterial genera correlated positively with serum androgen concentrations and negatively with estradiol levels.
The diversity and composition of the gut microbiota of young adults are influenced by the combined effects of sex, sex hormone concentrations, and obesity, presenting with specific abnormalities in women with PCOS.
肠道微生物群通过影响生理、代谢、营养和免疫功能，在健康和疾病中发挥重要作用。
评估多囊卵巢综合征（PCOS）女性的肠道微生物群组成，重点关注性别、性激素和肥胖对所发现关联的影响。
横断面研究。
学术医院。
我们招募了 15 名 PCOS 女性、16 名非高雄激素对照组女性和 15 名对照组男性。根据体重指数（BMI），参与者分为非肥胖（<30 kg/m2）或肥胖（≥30 kg/m2）。
连续 3 天进行饮食标准化（每天至少 300 克碳水化合物），然后进行粪便采样和标准的 75 克口服葡萄糖耐量试验。
通过 MiSeq 平台（Illumina）对 16S 核糖体 DNA 扩增子进行大规模测序，分析肠道微生物群的细菌丰度和组成。
与男性相比，女性的α细菌多样性降低，特别是肥胖的 PCOS 女性β多样性降低。PCOS 女性的肠道微生物群存在特定的异常，包括 Catenibacterium 和 Kandleria 属的丰度增加。当将所有参与者作为一个整体考虑时，细菌多样性指数和几个细菌属的丰度与血清雄激素浓度呈正相关，与雌二醇水平呈负相关。
年轻成年人的肠道微生物群多样性和组成受性别、性激素浓度和肥胖的综合影响，PCOS 女性表现出特定的异常。
