College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Daqing 163319, China.
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Int J Biol Macromol. 2021 Mar 1;172:550-559. doi: 10.1016/j.ijbiomac.2021.01.088. Epub 2021 Jan 16.
The crude polysaccharide was extracted from A. asphodeloides rhizomes and further purified to produce two fractions F (50.0%) and F (19.6%). The chemical constitutions of the polysaccharides were neutral sugars (51.4%-89.7%), uronic acids (1.0%-30.2%) and sulfate esters (3.4%-8.1%), with various ratios of monosaccharides including rhamnose (1.4%-6.1%), arabinose (7.1%-21.2%), xylose (0.2%-4.8%), mannose (39.9%-79.0%), glucose (6.0%-11.1%) and galactose (2.6%-22.0%). The molecular properties of the polysaccharides were investigated by the HPSEC-UV-MALLS-RI system, revealing the M 130.0 × 10-576.5 × 10 g/moL, R 87.6-382.6 nm and SV 0.3-54.3 cm/g. The polysaccharides stimulated RAW264.7 cells to produce considerable amounts of NO and up-regulate the expression of TNF-α, IL-1 and COX-2 genes. Polysaccharides exhibited the growth inhibitory effects on cancer cells lines of AGS, MKN-28 and MKN-45, in which F fraction exhibited prominent bioactivities. The AGS cells treated with F experienced condensed cytoplasm, shrinkage of nucleus and chromatin marginalization with the highest number of cells at early-stage apoptosis reaching 54.6%. The inhibitory effect of F polysaccharide on AGS cells was through MAPKs and STAT3 signaling pathways. The backbone of the F was mainly linked by (1 → 4)-linked mannopyranosyl and (1 → 3)-linked galactopyranosyl. Taken together, the polysaccharide from A. asphodeloides rhizomes could be utilized as medicinal, pharmacological and functional food ingredients.
从海韭菜根茎中提取粗多糖，并进一步纯化得到两个部分 F（50.0%）和 F（19.6%）。多糖的化学组成包括中性糖（51.4%-89.7%）、糖醛酸（1.0%-30.2%）和硫酸酯（3.4%-8.1%），单糖比例各不相同，包括鼠李糖（1.4%-6.1%）、阿拉伯糖（7.1%-21.2%）、木糖（0.2%-4.8%）、甘露糖（39.9%-79.0%）、葡萄糖（6.0%-11.1%）和半乳糖（2.6%-22.0%）。多糖的分子性质通过 HPSEC-UV-MALLS-RI 系统进行了研究，结果表明其重均分子量为 130.0×10-576.5×10 g/mol，瑞利系数为 87.6-382.6nm，比体积为 0.3-54.3cm/g。多糖刺激 RAW264.7 细胞产生大量的 NO，并上调 TNF-α、IL-1 和 COX-2 基因的表达。多糖对 AGS、MKN-28 和 MKN-45 癌细胞系具有生长抑制作用，其中 F 部分表现出显著的生物活性。F 处理的 AGS 细胞出现细胞质浓缩、细胞核缩小和染色质边缘化，早期凋亡的细胞数量最多，达到 54.6%。F 多糖对 AGS 细胞的抑制作用是通过 MAPKs 和 STAT3 信号通路实现的。F 多糖的主链主要由（1→4）-连接的甘露吡喃糖基和（1→3）-连接的半乳糖吡喃糖基组成。综上所述，海韭菜根茎多糖可作为药用、药理和功能性食品成分。
