Neuroscience Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran; Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
MSc in Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
J Ethnopharmacol. 2021 Jun 12;273:113826. doi: 10.1016/j.jep.2021.113826. Epub 2021 Jan 16.
Cuscuta epithymum Murr. (CE) is a parasitic plant used as a traditional medicine to treat various diseases such as muscle and joint pains and headache different parts of the world, Europe in the north, Asia in the east.
In this study, we aimed to investigate the anti-nociceptive effect of the methanolic extract of the aerial parts of CE and its probable mechanism(s) in mice.
The anti-nociceptive activity of different doses of CE methanolic extract (2.5, 5, 10, 25, 50 and 100 mg/kg, i.p.) was assessed using tail flick, formalin and writhing tests. Morphine (5 mg/kg, s.c.) was used as positive control drug. The possible mechanisms were evaluated by using naloxone (4 mg/kg, i.p.), ondansetron (4 mg/kg, i.p.), picrotoxin (0.6 mg/kg, i.p.) and MK-801 (0.03 mg/kg, i.p.).
GC-MS analysis indicated that one of the main components of CE extract was terpenoid compounds. The CE extract (25-100 mg/kg), like morphine, reduced tail flick latency and nociceptive response in both phases of the formalin test. We also observed that the extract significantly decreased the number of abdominal contractions dose-dependently from 5 to 100 mg/kg. The results of tail flick and the first phase of formalin test proved that unlike ondansetron and MK-801, naloxone and picotroxin were able to reverse the anti-nociceptive effect of CE extract.
Our observations showed the anti-nociceptive potential of the CE extract, which may be mediated by opioidergic and GABAergic systems.
菟丝子（CE）是一种寄生植物，被用作传统药物，用于治疗世界各地的各种疾病，如肌肉和关节疼痛以及头痛，包括欧洲北部和亚洲东部。
在这项研究中，我们旨在研究 CE 地上部分甲醇提取物的抗伤害作用及其在小鼠中的可能机制。
使用尾巴闪烁、福马林和扭体试验评估不同剂量的 CE 甲醇提取物（2.5、5、10、25、50 和 100mg/kg，ip）的抗伤害活性。吗啡（5mg/kg，sc）用作阳性对照药物。通过使用纳洛酮（4mg/kg，ip）、昂丹司琼（4mg/kg，ip）、戊四氮（0.6mg/kg，ip）和 MK-801（0.03mg/kg，ip）来评估可能的机制。
GC-MS 分析表明，CE 提取物的主要成分之一是萜类化合物。CE 提取物（25-100mg/kg）与吗啡相似，可延长尾巴闪烁潜伏期并减轻福尔马林测试的两个阶段的伤害反应。我们还观察到，提取物显著减少了剂量依赖性的腹部收缩次数，从 5 到 100mg/kg。尾巴闪烁和福马林测试第一阶段的结果表明，与昂丹司琼和 MK-801 不同，纳洛酮和戊四氮能够逆转 CE 提取物的抗伤害作用。
我们的观察结果表明，CE 提取物具有抗伤害作用，这可能是通过阿片能和 GABA 能系统介导的。
