Jaworska Aleksandra, Malek Kamilla, Kudelski Andrzej
Faculty of Chemistry, University of Warsaw, 1 Pasteur St., 02-093 Warsaw, Poland.
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15;251:119410. doi: 10.1016/j.saa.2020.119410. Epub 2020 Dec 31.
The value of pH in various parts of protoplasm can affect nearly all aspects of cell functions. Therefore, the determination of intracellular acid-base features is required in many areas of biological and biochemical studies. Because of a significant scientific importance of in vivo intracellular pH measurements, various groups carried out such experiments. In this review article we describe intracellular pH measurements using two the most sensitive optical spectroscopies: surface-enhanced Raman scattering (SERS) and fluorescence. It is reasonable to present these two techniques in one review article because the experimental approach in Raman and fluorescence experiments is relatively similar. The basic theoretical background explaining the mechanism of operation of fluorescence and SERS sensors are discussed and the motivations to carry out intracellular pH measurements are briefly described. Future perspectives in this field are also discussed.
原生质不同部位的pH值会影响细胞功能的几乎所有方面。因此，在生物学和生物化学研究的许多领域都需要测定细胞内酸碱特征。由于体内细胞内pH测量具有重大的科学意义，多个研究团队开展了此类实验。在这篇综述文章中，我们描述了使用两种最灵敏的光学光谱技术进行细胞内pH测量：表面增强拉曼散射（SERS）和荧光。将这两种技术放在一篇综述文章中介绍是合理的，因为拉曼和荧光实验的实验方法相对相似。文中讨论了解释荧光和SERS传感器工作机制的基本理论背景，并简要描述了进行细胞内pH测量的动机。此外，还讨论了该领域的未来前景。
