Instituto de Investigación e Ingeniería Ambiental, IIIA, UNSAM, CONICET, 3iA. Campus Miguelete, 25 de Mayo y Francia, C.P. 1650, San Martín, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Instituto de Investigación e Ingeniería Ambiental, IIIA, UNSAM, CONICET, 3iA. Campus Miguelete, 25 de Mayo y Francia, C.P. 1650, San Martín, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Chemosphere. 2021 May;271:129598. doi: 10.1016/j.chemosphere.2021.129598. Epub 2021 Jan 11.
In situ bioassays provide valuable information about the environment and offer more realistic results than usual laboratory experiments. The aim of this study was to evaluate the quality of water bodies from the lower Paraná River basin, the second most important in South America, through analysis of physiochemical parameters, metals and pesticides and in situ exposure of Rhinella fernandezae larvae to assess oxidative stress biomarkers. The sites were: S1(Morejón stream, reference); S2, S3(De la Cruz stream upstream and downstream, respectively) and S4(Arrecifes River). In all sites, dissolved oxygen was low, atrazine was detected and Cu was higher than the limit for aquatic life protection. According to the water quality index, S2, S3 and S4 presented bad water quality, while S1 good water quality. Larvae were exposed in situ for 96h in order to analyze: lipid peroxidation(TBARS) as oxidative damage, antioxidant enzymatic (catalase-CAT-, superoxide dismutase-SOD- and glutathione s-transferase-GST-) and non-enzymatic defenses (reduced glutathione-GSH-). Larvae exposed in the most impacted sites (S2, S3 and S4) presented oxidative stress since the levels of TBARS were around 2 times higher than in S1. Also, the other oxidative stress biomarkers were altered in larvae exposed at S2, S3 and S4. These results highlight the importance of analyzing oxidative stress biomarkers during in situ exposures since they are useful tools for documenting the extent of exposure at sublethal levels. The complex pollution of the water bodies affected the exposed larvae, which may jeopardize the native populations.
原位生物测定为环境提供了有价值的信息，并提供比常规实验室实验更现实的结果。本研究旨在通过分析生理化学参数、金属和农药以及 Rhinella fernandezae 幼虫的原位暴露来评估南美洲第二大的巴拉那河下游流域水体的质量，以评估氧化应激生物标志物。研究地点为：S1（Morejón 溪流，参照点）；S2、S3（分别为德拉萨克鲁斯溪流的上游和下游）和 S4（Arrecifes 河）。在所有地点，溶解氧都很低，检测到莠去津，铜含量高于水生生物保护的限值。根据水质指数，S2、S3 和 S4 水质较差，而 S1 水质较好。为了分析：脂质过氧化（TBARS）作为氧化损伤、抗氧化酶（过氧化氢酶-CAT-、超氧化物歧化酶-SOD-和谷胱甘肽 s-转移酶-GST-）和非酶防御（还原型谷胱甘肽-GSH-），将幼虫在原位暴露 96 小时。在受影响最严重的地点（S2、S3 和 S4）暴露的幼虫出现氧化应激，因为 TBARS 的水平比 S1 高约 2 倍。此外，暴露于 S2、S3 和 S4 的幼虫的其他氧化应激生物标志物也发生了改变。这些结果强调了在原位暴露期间分析氧化应激生物标志物的重要性，因为它们是在亚致死水平记录暴露程度的有用工具。受污染水体的复杂污染影响了暴露的幼虫，这可能危及当地的种群。
