In situ exposure of amphibian larvae (Rhinella fernandezae) to assess water quality by means of oxidative stress biomarkers in water bodies with different anthropic influences.

In situ bioassays provide valuable information about the environment and offer more realistic results than usual laboratory experiments. The aim of this study was to evaluate the quality of water bodies from the lower Paraná River basin, the second most important in South America, through analysis of physiochemical parameters, metals and pesticides and in situ exposure of Rhinella fernandezae larvae to assess oxidative stress biomarkers. The sites were: S1(Morejón stream, reference); S2, S3(De la Cruz stream upstream and downstream, respectively) and S4(Arrecifes River). In all sites, dissolved oxygen was low, atrazine was detected and Cu was higher than the limit for aquatic life protection. According to the water quality index, S2, S3 and S4 presented bad water quality, while S1 good water quality. Larvae were exposed in situ for 96h in order to analyze: lipid peroxidation(TBARS) as oxidative damage, antioxidant enzymatic (catalase-CAT-, superoxide dismutase-SOD- and glutathione s-transferase-GST-) and non-enzymatic defenses (reduced glutathione-GSH-). Larvae exposed in the most impacted sites (S2, S3 and S4) presented oxidative stress since the levels of TBARS were around 2 times higher than in S1. Also, the other oxidative stress biomarkers were altered in larvae exposed at S2, S3 and S4. These results highlight the importance of analyzing oxidative stress biomarkers during in situ exposures since they are useful tools for documenting the extent of exposure at sublethal levels. The complex pollution of the water bodies affected the exposed larvae, which may jeopardize the native populations.