Lee J C, Perez N E, Hopkins C A, Pier G B
Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts 02115.
J Infect Dis. 1988 Apr;157(4):723-30. doi: 10.1093/infdis/157.4.723.
In this study, we determined that immunization with capsular polysaccharide from Staphylococcus aureus could protect mice against nonlethal infections induced by encapsulated staphylococci. We immunized mice with either formalin-killed bacteria or purified capsular polysaccharide (PCP) and challenged them with one of three related S. aureus strains that varied in capsule size. Quantitative cultures of blood and kidney from the animals were performed to evaluate protection. Immunization with whole bacteria protected mice against infection with the homologous strain. Mice immunized with PCP were protected when challenged intravenously with either a highly encapsulated S. aureus strain or a microencapsulated mutant but not with an unencapsulated mutant. Protection correlated with capsular antibody levels in the immunized animals. Immunity to staphylococcal infection could be passively transferred to naive animals by using immune serum. These experiments suggest that the S. aureus capsular polysaccharide merits further study as a potential vaccine candidate for preventing staphylococcal infection.
在本研究中，我们确定用金黄色葡萄球菌的荚膜多糖进行免疫可保护小鼠免受由包膜葡萄球菌引起的非致命性感染。我们用福尔马林灭活的细菌或纯化的荚膜多糖（PCP）免疫小鼠，并用三种相关的金黄色葡萄球菌菌株之一对其进行攻击，这三种菌株的荚膜大小各不相同。对动物的血液和肾脏进行定量培养以评估保护效果。用全菌免疫可保护小鼠免受同源菌株的感染。用PCP免疫的小鼠在静脉注射高度包膜化的金黄色葡萄球菌菌株或微包膜化突变体时受到保护，但用无包膜突变体攻击时则不受保护。保护作用与免疫动物体内的荚膜抗体水平相关。通过使用免疫血清，对葡萄球菌感染的免疫力可被动转移至未免疫的动物。这些实验表明，金黄色葡萄球菌荚膜多糖作为预防葡萄球菌感染的潜在疫苗候选物值得进一步研究。
