McKenney D, Pouliot K L, Wang Y, Murthy V, Ulrich M, Döring G, Lee J C, Goldmann D A, Pier G B
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Department of Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Science. 1999 May 28;284(5419):1523-7. doi: 10.1126/science.284.5419.1523.
Vaccines based on preferential expression of bacterial antigens during human infection have not been described. Staphylococcus aureus synthesized poly-N-succinyl beta-1-6 glucosamine (PNSG) as a surface polysaccharide during human and animal infection, but few strains expressed PNSG in vitro. All S. aureus strains examined carried genes for PNSG synthesis. Immunization protected mice against kidney infections and death from strains that produced little PNSG in vitro. Nonimmune infected animals made antibody to PNSG, but serial in vitro cultures of kidney isolates yielded mostly cells that did not produce PNSG. PNSG is a candidate for use in a vaccine to protect against S. aureus infection.
基于细菌抗原在人类感染期间优先表达的疫苗尚未见报道。金黄色葡萄球菌在人类和动物感染期间合成聚-N-琥珀酰-β-1,6-葡糖胺（PNSG）作为表面多糖，但很少有菌株在体外表达PNSG。所有检测的金黄色葡萄球菌菌株都携带PNSG合成基因。免疫可保护小鼠免受肾脏感染，并防止因体外产生少量PNSG的菌株导致的死亡。未免疫的感染动物产生了针对PNSG的抗体，但肾脏分离株的连续体外培养大多产生不产生PNSG的细胞。PNSG是一种可用于预防金黄色葡萄球菌感染的疫苗候选物。
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