Guzman-Puyol Susana, Heredia-Guerrero José A, Ceseracciu Luca, Hajiali Hadi, Canale Claudio, Scarpellini Alice, Cingolani Roberto, Bayer Ilker S, Athanassiou Athanassia, Mele Elisa
DIBRIS, University of Genoa, Via Opera Pia, 13, Genova 16145, Italy.
ACS Biomater Sci Eng. 2016 Apr 11;2(4):526-534. doi: 10.1021/acsbiomaterials.5b00500. Epub 2016 Mar 24.
Here, we show the production of nanofibrous mats with controlled mechanical properties and excellent biocompatibility by combining fibroin with pure cellulose and cellulose-rich parsley powder agro-waste. To this end, trifluoroacetic acid was used as a common solvent for all of the involved biomaterials, achieving highly homogeneous blends that were suitable for the electrospinning technique. Morphological analysis revealed that the electrospun composite nanofibers were well-defined and defect-free, with a diameter in the range of 65-100 nm. Mechanical investigations demonstrated that the fibrous mats exhibited an increased stiffness when pure fibroin was combined with cellulose, whereas they possessed an increased flexibility when the parsley waste was added to fibroin. Lastly, the produced mats were highly biocompatible, as demonstrated by the promoted proliferation of fibroblast cells. The characteristics of the hybrid fibroin-cellulose nanofibers, in terms of nanoscale topography, mechanical properties, and biocompatibility, are attractive and potentially applicable in the biomedical sector.
在此，我们展示了通过将丝素蛋白与纯纤维素以及富含纤维素的欧芹粉农业废料相结合，制备出具有可控机械性能和优异生物相容性的纳米纤维垫。为此，三氟乙酸被用作所有相关生物材料的通用溶剂，从而获得了适用于静电纺丝技术的高度均匀的混合物。形态分析表明，静电纺复合纳米纤维轮廓清晰且无缺陷，直径在65 - 100纳米范围内。力学研究表明，当纯丝素蛋白与纤维素结合时，纤维垫的刚度增加，而当将欧芹废料添加到丝素蛋白中时，纤维垫具有更高的柔韧性。最后，如成纤维细胞增殖得到促进所示，所制备的垫子具有高度生物相容性。混合丝素蛋白 - 纤维素纳米纤维在纳米级形貌、机械性能和生物相容性方面的特性具有吸引力，并且有可能应用于生物医学领域。
