The Pangenomics Lab, School of Science, RMIT University, Melbourne, VIC 3083, Australia.
Vietnam Institute of Agricultural Engineering and Post-Harvest Technology, Hanoi 10000, Vietnam.
Nutrients. 2021 Jan 5;13(1):151. doi: 10.3390/nu13010151.
Manuka honey and newly developed honeys (arjuna, guggul, jiaogulan and olive) were examined for their physicochemical, biochemical properties and effects on oxidative stress and cholesterol homeostasis in fatty acid-induced HepG2 cells. The honeys exhibited standard moisture content (<20%), electrical conductivity (<0.8 mS/cm), acidic pH, and monosaccharides (>60%), except olive honey (<60% total monosaccharides). They all expressed non-Newtonian behavior and 05 typical regions of the FTIR spectra as those of natural ones. Guggul and arjuna, manuka honeys showed the highest phenolic contents, correlating with their significant antioxidant activities. Arjuna, guggul and manuka honeys demonstrated the agreement of total cholesterol reduction and the transcriptional levels of , , , , . Jiaogulan honey showed the least antioxidant content and activity, but it was the most cytotoxic. Both jiaogulan and olive honeys modulated the tested gene in the pattern that should lead to a lower TC content, but this reduction did not occur after 24 h. All 2% concentrations of tested honeys elicited a clearer effect on gene expression. In conclusion, the new honeys complied with international norms for natural honeys and we provide partial evidence for the protective effects of manuka, arjuna and guggul honeys amongst the tested ones on key biomarkers of oxidative stress and cholesterol homeostasis, pending further studies to better understand their modes of action.
麦卢卡蜂蜜和新开发的蜂蜜（南非醉茄、乳香、绞股蓝和橄榄油）的物理化学、生化特性以及对脂肪酸诱导的 HepG2 细胞氧化应激和胆固醇稳态的影响进行了研究。这些蜂蜜的水分含量（<20%）、电导率（<0.8 mS/cm）、酸性 pH 值和单糖含量（>60%）均符合标准，除橄榄油蜂蜜（总单糖含量<60%）外。它们都表现出非牛顿行为和 05 个典型的 FTIR 光谱区域，与天然蜂蜜相似。乳香、南非醉茄和麦卢卡蜂蜜的酚类含量最高，与其显著的抗氧化活性相关。南非醉茄、乳香和麦卢卡蜂蜜的总胆固醇降低和基因转录水平的表达呈一致趋势，表明它们具有降低胆固醇的作用。绞股蓝蜂蜜的抗氧化含量和活性最低，但细胞毒性最大。绞股蓝和橄榄油蜂蜜都以一种应该导致 TC 含量降低的模式调节了测试基因，但这种降低在 24 小时后并未发生。所有 2%浓度的测试蜂蜜都对基因表达产生了更明显的影响。综上所述，新蜂蜜符合天然蜂蜜的国际标准，我们提供了部分证据表明，在测试的蜂蜜中，麦卢卡、南非醉茄和乳香蜂蜜对氧化应激和胆固醇稳态的关键生物标志物具有保护作用，但需要进一步研究以更好地了解它们的作用模式。
