Université de Nantes, CHU Nantes, CNRS, INSERM, L'institut du Thorax, F-44000 Nantes, France.
Int J Mol Sci. 2021 Jan 14;22(2):774. doi: 10.3390/ijms22020774.
The use of animal models in fundamental or pre-clinical research remains an absolute requirement for understanding human pathologies and developing new drugs. In order to transpose these results into clinical practice, many parameters must be taken into account to limit bias. Attention has recently been focused on the sex, age or even strain of each animal, but the impact of diet has been largely neglected. Soy, which is commonly used in the diet in varying quantities can affect their physiology. In order to assess whether the presence of soy can impact the obtained results, we studied the impact of a soy-based diet a soy-free diet, on diastolic function in a rat model based on transgenic overexpression of the β-adrenergic receptors in the endothelium and characterized by the appearance of diastolic dysfunction with age. Our results show that the onset of diastolic dysfunction is only observed in transgenic male rats fed with a soy-free diet in the long term. Our study highlights the importance of the diet's choice in the study design process, especially regarding the proportion of soy, to correctly interpret the outcome as low-cost diets are more likely to be highly concentrated in soy.
在基础或临床前研究中使用动物模型仍然是理解人类病理学和开发新药的绝对要求。为了将这些结果转化为临床实践，必须考虑许多参数来限制偏差。最近，人们关注的焦点是每种动物的性别、年龄甚至品系，但饮食的影响在很大程度上被忽视了。大豆在饮食中以不同的量被广泛使用，可能会影响它们的生理机能。为了评估大豆的存在是否会影响获得的结果，我们研究了以大豆为基础的饮食和无大豆饮食对基于内皮β-肾上腺素能受体转基因过表达的大鼠模型舒张功能的影响，该模型的特征是随着年龄的增长出现舒张功能障碍。我们的结果表明，只有长期食用无大豆饮食的转基因雄性大鼠才会出现舒张功能障碍。我们的研究强调了在研究设计过程中选择饮食的重要性，特别是关于大豆的比例，以正确解释结果，因为低成本饮食更有可能高度集中在大豆中。
