Department of Applied Chemistry, School of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang 524088, China.
Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang 524023, China.
Molecules. 2021 Jan 16;26(2):452. doi: 10.3390/molecules26020452.
Chitosan is the only cationic polysaccharide found in nature. It has broad application prospects in biomaterials, but its application is limited due to its poor solubility in water. A novel chitosan derivative was synthesized by amidation of chitosan with 18β-glycyrrhetinic acid and sialic acid. The chitosan derivatives were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and measurement of the zeta potential. We also investigated the solubility, cytotoxicity, and blood compatibility of chitosan derivatives. 18β-glycyrrhetinic acid and sialic acid could be grafted onto chitosan molecular chains. The thermal stability of the synthesized chitosan derivatives was decreased and the surface was positively charged in water and phosphate-buffered saline. After chitosan had been modified by 18 β-glycyrrhetinic acid and sialic acid, the solubility of chitosan was improved greatly in water and phosphate-buffered saline, and percent hemolysis was <5%. Novel amphiphilic chitosan derivatives could be suitable polymers for biomedical purposes.
壳聚糖是自然界中唯一存在的阳离子多糖。它在生物材料中有广泛的应用前景，但由于其在水中的溶解度差，其应用受到限制。通过壳聚糖与 18β-甘草次酸和唾液酸的酰胺化反应合成了一种新型壳聚糖衍生物。通过傅里叶变换红外光谱、热重分析和zeta 电位测量对壳聚糖衍生物进行了表征。我们还研究了壳聚糖衍生物的溶解度、细胞毒性和血液相容性。18β-甘草次酸和唾液酸可以接枝到壳聚糖分子链上。合成的壳聚糖衍生物的热稳定性降低，在水和磷酸盐缓冲盐溶液中表面带正电荷。壳聚糖经过 18β-甘草次酸和唾液酸修饰后，在水和磷酸盐缓冲盐溶液中的溶解度大大提高，溶血率<5%。新型两亲性壳聚糖衍生物可能是适合生物医学用途的聚合物。
