Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, 555 35 Thessaloniki, Greece.
Department of Chemistry, University of Ioannina, P.O. Box 1186, 45110 Ioannina, Greece.
Mar Drugs. 2021 Jan 15;19(1):36. doi: 10.3390/md19010036.
In the present study, chitosan (CS) and pectin (PEC) were utilized for the preparation of 3D printable inks through pneumatic extrusion for biomedical applications. CS is a polysaccharide with beneficial properties; however, its printing behavior is not satisfying, rendering the addition of a thickening agent necessary, i.e., PEC. The influence of PEC in the prepared inks was assessed through rheological measurements, altering the viscosity of the inks to be suitable for 3D printing. 3D printing conditions were optimized and the effect of different drying procedures, along with the presence or absence of a gelating agent on the CS-PEC printed scaffolds were assessed. The mean pore size along with the average filament diameter were measured through SEM micrographs. Interactions among the characteristic groups of the two polymers were evident through FTIR spectra. Swelling and hydrolysis measurements confirmed the influence of gelation and drying procedure on the subsequent behavior of the scaffolds. Ascribed to the beneficial pore size and swelling behavior, fibroblasts were able to survive upon exposure to the ungelated scaffolds.
在本研究中，壳聚糖（CS）和果胶（PEC）通过气动挤压被用于制备 3D 可打印墨水，用于生物医学应用。CS 是一种具有有益特性的多糖；然而，其打印性能并不令人满意，因此需要添加增稠剂，即 PEC。通过流变学测量评估了 PEC 在制备墨水中的影响，改变了墨水的粘度以适用于 3D 打印。优化了 3D 打印条件，并评估了不同干燥程序的影响，以及是否存在胶凝剂对 CS-PEC 打印支架的影响。通过 SEM 显微照片测量了平均孔径和平均丝径。FTIR 光谱表明两种聚合物的特征基团之间存在相互作用。溶胀和水解测量证实了胶凝和干燥程序对支架后续行为的影响。由于有益的孔径和溶胀行为，未胶凝支架能够使成纤维细胞存活。
