Nicault Matthieu, Zaiter Ali, Dumarcay Stéphane, Chaimbault Patrick, Gelhaye Eric, Leblond Pierre, Bontemps Cyril
Université de Lorraine, INRAE, DynAMic, F-54000 Nancy, France.
Université de Lorraine, INRAE, IAM, F-54000 Nancy, France.
Microorganisms. 2021 Jan 15;9(1):178. doi: 10.3390/microorganisms9010178.
The bacteria of the genus and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventing the full metabolic potential of these organisms from being exploited. Because it generates biotic cues encountered during natural growth conditions, co-culture is a means to elicit such cryptic compounds. In this study, we explored 72 different -fungus interaction zones (SFIZs) generated during the co-culture of eight and nine fungi. Two SFIZs were selected because they showed an elicitation of anti-bacterial activity compared to mono-cultures. The study of these SFIZs showed that co-culture had a strong impact on the metabolic expression of each partner and enabled the expression of specific compounds. These results show that mimicking the biotic interactions present in this ecological niche is a promising avenue of research to explore the metabolic capacities of and fungi.
该属细菌和担子菌真菌含有许多生物合成基因簇（BGCs），这些基因簇是许多具有医学或工业价值的生物活性分子的起源。然而，大多数BGCs在标准实验室生长条件下不表达，这使得这些生物的全部代谢潜力无法得到开发。由于共培养会产生自然生长条件下遇到的生物线索，因此它是引发此类隐性化合物的一种手段。在本研究中，我们探索了八种细菌和九种真菌共培养过程中产生的72个不同的细菌-真菌相互作用区域（SFIZs）。选择了两个SFIZs，因为与单培养相比，它们表现出抗菌活性的激发。对这些SFIZs的研究表明，共培养对每个伙伴的代谢表达有强烈影响，并能使特定化合物表达。这些结果表明，模拟这个生态位中存在的生物相互作用是探索细菌和真菌代谢能力的一个有前途的研究途径。
