Laboratory of Aquatic Animal Diseases, Research Institute of Natural Science, College of Veterinary Medicine, Gyeongsang National University, 501-201, 501, Jinju-daero, Jinju-si 52828, Korea.
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK.
Int J Mol Sci. 2021 Jan 15;22(2):847. doi: 10.3390/ijms22020847.
In higher vertebrates, helper and cytotoxic T cells, referred to as CD4 and CD8 T lymphocytes, respectively, are mainly associated with adaptive immunity. The adaptive immune system in teleosts involves T cells equivalent to those found in mammals. We previously generated monoclonal antibodies (mAbs) against olive flounder () CD4 T cells, CD4-1 and CD4-2, and used these to describe the olive flounder's CD4 Tcell response during a viral infection. In the present study, we successfully produced mAbs against CD8 T lymphocytes and their specificities were confirmed using immuno-blotting, immunofluorescence staining, flow cytometry analysis andreverse transcription polymerase chain reaction (RT-PCR). The results showed that these mAbs are specific for CD8 T lymphocytes. We also investigated variations in CD4 and CD8 T cells populations, and analyzed the expression of immune-related genes expressed by these cells in fish infected with nervous necrosis virus or immunized with thymus dependent and independent antigens. We found that both CD4 and CD8 T lymphocyte populations significantly increased in these fish and Th1-related genes were up-regulated compared to the control group. Collectively, these findings suggest that the CD4 and CD8 T lymphocytes in olive flounder are similar to the helper and cytotoxic T cells found in mammals, and Th1 and cytotoxic immune responses are primarily involved in the early adaptive immune response against extracellular antigens.
在高等脊椎动物中，辅助性 T 细胞和细胞毒性 T 细胞分别被称为 CD4 和 CD8 T 淋巴细胞，主要与适应性免疫有关。鱼类的适应性免疫系统涉及到类似于哺乳动物中发现的 T 细胞。我们之前生成了针对牙鲆 CD4 T 细胞的单克隆抗体 (mAb)，即 CD4-1 和 CD4-2，并利用这些抗体描述了牙鲆在病毒感染期间的 CD4 T 细胞反应。在本研究中，我们成功地制备了针对 CD8 T 淋巴细胞的 mAb，并通过免疫印迹、免疫荧光染色、流式细胞术分析和逆转录聚合酶链反应 (RT-PCR) 确认了其特异性。结果表明，这些 mAb 特异性识别 CD8 T 淋巴细胞。我们还研究了 CD4 和 CD8 T 细胞群体的变化，并分析了感染神经坏死病毒或用胸腺依赖性和非依赖性抗原免疫的鱼类中这些细胞表达的免疫相关基因。结果显示，与对照组相比，这些鱼类的 CD4 和 CD8 T 淋巴细胞群体显著增加，并且 Th1 相关基因上调。综上所述，这些发现表明，牙鲆的 CD4 和 CD8 T 淋巴细胞类似于哺乳动物中发现的辅助性 T 细胞和细胞毒性 T 细胞，并且 Th1 和细胞毒性免疫反应主要参与针对细胞外抗原的早期适应性免疫反应。
