Yadav Ashvini K, Bhooshan Suneel, Johnson Allen, Asati Dinesh P, Nema Shashwati, Biswas Debasis
Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhopal, Madhya Pradesh, India.
Department of Dermatology, All India Institute of Medical Sciences (AIIMS), Bhopal, Madhya Pradesh, India.
J Lab Physicians. 2020 Dec;12(4):233-238. doi: 10.1055/s-0040-1720943. Epub 2020 Dec 30.
( ) is an emerging pathogen that is highly resistant to antibiotics and is capable of causing persistent infections that are difficult to treat. Acne vulgaris patients visiting dermatology OPD of our tertiary care hospital during the study period of 2 months were recruited. Skin swabs were collected, and the sample was processed on 5% sheep-blood agar for anaerobic culture by the GasPak method. Isolates were identified by the standard biochemical test. Antimicrobial susceptibility testing was performed for clinically relevant antibiotics by the E-strip method. The clinical response was evaluated after 1-month follow-up to the prescribed antibiotics. Minocycline, doxycycline, ceftriaxone, and tetracycline were the most effective antibiotics. Nonsusceptibility to clindamycin and erythromycin were observed in 11.9% and 31% isolates, respectively, with 9.5% isolates being nonsusceptible to both. For none of the antibiotics we found significant difference in the proportion of susceptible and nonsusceptible isolates between mild, moderate, and severe grades of acne vulgaris. For none of the antibiotic regimens, significant difference was observed between nonresponders and responders. Twenty-seven patients received clindamycin and among them 16 of 19 responders and 6 of 8 nonresponders yielded growth of clindamycin-susceptible isolates ( = 0.57). We observed significant prevalence of resistant strains of among patients with acne vulgaris. No association was observed between in vitro susceptibility results and treatment outcome.
（ ）是一种新兴病原体，对抗生素具有高度抗性，能够引起难以治疗的持续性感染。在为期2个月的研究期间，招募了到我们三级护理医院皮肤科门诊就诊的寻常痤疮患者。采集皮肤拭子，样本通过GasPak方法在5%羊血琼脂上进行厌氧培养。通过标准生化试验鉴定分离株。采用E试纸条法对临床相关抗生素进行药敏试验。在对规定抗生素进行1个月随访后评估临床反应。
米诺环素、多西环素、头孢曲松和四环素是最有效的抗生素。分别有11.9%和31%的分离株对克林霉素和红霉素不敏感，9.5%的分离株对两者均不敏感。对于我们所研究的任何一种抗生素，寻常痤疮轻度、中度和重度分级之间敏感和不敏感分离株的比例均无显著差异。对于任何一种抗生素治疗方案，无反应者和有反应者之间均未观察到显著差异。27例患者接受了克林霉素治疗，其中19例有反应者中的16例和8例无反应者中的6例培养出对克林霉素敏感的分离株（ = 0.57）。
我们观察到寻常痤疮患者中（ ）耐药菌株的显著流行。体外药敏结果与治疗结果之间未观察到相关性。
