Ruchiatan Kartika, Rizqandaru Trustia, Satjamanggala Panji Respati, Tache Nisrina, Cahyadi Adi Imam, Rezano Andri, Gunawan Hendra, Sutedja Eva Krishna, Dwiyana Reiva Farah, Hidayah Risa Miliawati Nurul, Achdiat Pati Aji, Sutedja Endang, Suwarsa Oki, Hindritiani Reti
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia.
Doctorate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
Clin Cosmet Investig Dermatol. 2023 Sep 11;16:2457-2465. doi: 10.2147/CCID.S422486. eCollection 2023.
Acne vulgaris (AV) is a common and chronic disorder of the pilosebaceous unit and has a multifactorial pathology, including activities of () and (). Antibiotic resistance has become a major concern in dermatology daily practice, and the ability of biofilm formation by both bacteria is suggested to increase antibiotic resistance in acne.
Our aim was to analyze the comparison of antibiotic resistance between biofilm-forming (BF) and non-biofilm-forming (NBF) strains of and towards seven antibiotics commonly used for acne.
This is a cross-sectional analytical study involving 60 patients with AV. Samples were obtained from closed comedones on the forehead using the standardized skin surface biopsy (SSSB) method at the Cosmetic Dermatology Clinic Dr. Hasan Sadikin in Bandung, Indonesia. Isolates were cultured and identified before undergoing the biofilm-forming test using the tissue culture plate method. Antibiotic susceptibility testing for each antibiotic was then performed using the disc diffusion method.
The incidence of antibiotic resistance to clindamycin in BF and NBF isolates was 54.5% (p=1.00), while in BF and NBF isolates, it was 54.5% and 45.5% respectively (p=0.67). The incidence of antibiotic resistance to erythromycin and azithromycin in BF and NBF isolates was 54.5% and 63.6% respectively (p=1.00), whereas for BF and NBF isolates, it was 54.5% (p=1.00). There was no resistance observed to tetracycline, doxycycline, levofloxacin, and cotrimoxazole in all groups.
There were no significant differences in resistance against seven antibiotics between the and in BF and NBF groups. Furthermore, although statistically not significant, some resistances were observed against clindamycin, erythromycin, and azithromycin. Consequently, the use of these three antibiotics should be judiciously regulated.
寻常痤疮（AV）是一种常见的毛囊皮脂腺慢性疾病，其病理机制具有多因素性，包括（）和（）的活性。抗生素耐药性已成为皮肤科日常临床工作中的一个主要问题，且这两种细菌形成生物膜的能力被认为会增加痤疮中的抗生素耐药性。
我们的目的是分析痤疮丙酸杆菌和金黄色葡萄球菌的生物膜形成（BF）菌株与非生物膜形成（NBF）菌株对七种常用于治疗痤疮的抗生素的耐药性比较。
这是一项横断面分析研究，涉及60例寻常痤疮患者。样本是在印度尼西亚万隆哈桑·萨迪金博士美容皮肤科诊所，采用标准化皮肤表面活检（SSSB）方法从前额闭合性粉刺中获取的。分离菌株在使用组织培养板法进行生物膜形成试验之前进行培养和鉴定。然后使用纸片扩散法对每种抗生素进行药敏试验。
痤疮丙酸杆菌BF和NBF分离株对克林霉素的耐药发生率为54.5%（p = 1.00），而金黄色葡萄球菌BF和NBF分离株的耐药发生率分别为54.5%和45.5%（p = 0.67）。痤疮丙酸杆菌BF和NBF分离株对红霉素和阿奇霉素的耐药发生率分别为54.5%和63.6%（p = 1.00），而金黄色葡萄球菌BF和NBF分离株的耐药发生率为54.5%（p = 1.00）。所有组对四环素、多西环素、左氧氟沙星和复方新诺明均未观察到耐药性。
BF和NBF组中痤疮丙酸杆菌和金黄色葡萄球菌对七种抗生素的耐药性无显著差异。此外，虽然在统计学上不显著，但观察到对克林霉素、红霉素和阿奇霉素存在一些耐药情况。因此，应谨慎规范使用这三种抗生素。
