Fu Xin, Zhang Li, Dan Li, Wang Ke, Xu Yue
Department of Gynecology Cancer, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and HospitalTianjin 300060, China.
Laboratory of Cancer Cell Biology, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and HospitalTianjin 300060, China.
Am J Transl Res. 2017 Sep 15;9(9):4094-4103. eCollection 2017.
Ovarian cancer ranks as the fifth common tumour in women and is the leading cause of cancer-related death among the women. Long non-coding RNAs (lncRNAs) play crucial roles in the development of tumors. However, the expression and the role of EWSAT1 in ovarian tumor are still unknown. In our study, we found the expression of EWSAT1 was upregulated in ovarian cancer cell lines and samples. Ecoptic expression of EWSAT1 suppressed miR-330-5p expression in ovarian cancer cell. In addition, the expression of miR-330-5p was downregulated in ovarian cancer cell lines and samples. Moreover, the expression of miR-330-5p was negatively related with the expression of EWSAT1 in ovarian cancer samples. Furthermore, we identified Pdia3 was a direct target gene of miR-330-5p. Ecoptic expression of miR-330-5p suppressed Pdia3 expression and overexpression of EWSAT1 promoted Pdia3 expression in ovarian cancer cell. In addition, Ecoptic expression of miR-330-5p promoted ovarian cancer cell proliferation, colony formation and invasion. Overexpression of EWSAT1 increased ovarian cancer cell proliferation, colony formation and invasion through targeting miR-330-5p. These data suggested that EWSAT1 might act as an oncogene in the development of ovarian cancer partly through inhibiting miR-330-5p expression.
卵巢癌是女性中第五大常见肿瘤，也是女性癌症相关死亡的主要原因。长链非编码RNA（lncRNA）在肿瘤发展中起关键作用。然而，EWSAT1在卵巢肿瘤中的表达及作用仍不清楚。在我们的研究中，我们发现EWSAT1在卵巢癌细胞系和样本中的表达上调。异位表达EWSAT1可抑制卵巢癌细胞中miR-330-5p的表达。此外，miR-330-5p在卵巢癌细胞系和样本中的表达下调。而且，在卵巢癌样本中miR-330-5p的表达与EWSAT1的表达呈负相关。此外，我们确定Pdia3是miR-330-5p的直接靶基因。异位表达miR-330-5p可抑制Pdia3表达，而EWSAT1的过表达可促进卵巢癌细胞中Pdia3的表达。此外，异位表达miR-330-5p可促进卵巢癌细胞增殖、集落形成和侵袭。EWSAT1的过表达通过靶向miR-330-5p增加卵巢癌细胞增殖、集落形成和侵袭。这些数据表明，EWSAT1可能部分通过抑制miR-330-5p的表达在卵巢癌发生过程中发挥癌基因作用。
