Sletvold O, Laerum O D, Riise T
Gade Institute, Department of Pathology, University of Bergen, Haukeland Hospital, Norway.
Mech Ageing Dev. 1988 Jan;42(1):91-104. doi: 10.1016/0047-6374(88)90065-6.
Non-proliferative and proliferative myeloid, and lymphoid and erythroid bone marrow cells were studied in aging female C3H mice. A chronobiological approach was used and mice aged 16, 21 and 26 months were examined vs. 3 month-old mice every 3 h during the 24-h period in three different experiments. Significant circadian fluctuations were observed in most of the cell populations, even in the oldest mice. The rhythmicity patterns might be different at different times of the year, and in young mice seasonal fluctuations in the 24-h mean values were observed. The absolute numbers of the 24-h means seemed to be highest at 21 months of age in all cell lines and maturation stages. Sinus function fitting indicated a decline of the amplitudes in aging mice. Minor age-related phase-differences were indicated in some populations. However, the fitting of original data to single sinus functions was variable and often obscured important features in the cell number variations. The present investigation illustrates the importance of performing time-sequence studies in hematology.
对衰老雌性C3H小鼠的非增殖性和增殖性髓系、淋巴系及红系骨髓细胞进行了研究。采用了时间生物学方法，在三个不同实验中，于24小时期间每3小时对16、21和26月龄的小鼠与3月龄小鼠进行检查。在大多数细胞群体中均观察到显著的昼夜波动，即使在最老龄的小鼠中也是如此。节律模式可能在一年中的不同时间有所不同，并且在年轻小鼠中观察到24小时平均值的季节性波动。所有细胞系和成熟阶段的24小时平均值的绝对数量似乎在21月龄时最高。正弦函数拟合表明衰老小鼠的振幅下降。在一些群体中显示出与年龄相关的微小相位差异。然而，将原始数据拟合到单个正弦函数的情况各不相同，并且常常掩盖了细胞数量变化中的重要特征。本研究说明了在血液学中进行时间序列研究的重要性。
