State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, South China Agricultural University, Guangzhou, China.
Methods Mol Biol. 2021;2238:19-35. doi: 10.1007/978-1-0716-1068-8_2.
Efficient stacking of multiple genes is a critical element in metabolic engineering of complex pathways, synthetic biology, and genetic improvement of complex agronomic traits in plants. Here we present a high-efficiency multigene assembly and transformation vector system, TransGene Stacking II (TGS II), for these purposes. The operation process is described in detail, and the successful operation mainly depends on effective reagents, special Escherichia coli strains, and basic molecular biological means without other specific equipments.
高效地堆叠多个基因是代谢工程复杂途径、合成生物学和植物复杂农艺性状遗传改良的关键要素。本文介绍了一种高效的多基因组装和转化载体系统 TransGene Stacking II（TGS II），用于这些目的。详细描述了操作过程，成功的操作主要依赖于有效的试剂、特殊的大肠杆菌菌株和基本的分子生物学手段，而不需要其他特殊设备。
