State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.
目标碱基编辑在植物中无需外源 DNA 供体或双链 DNA 切割，将加速广泛的作物的基因组修饰和育种。我们使用 CRISPR-Cas9 切口酶-胞嘧啶脱氨酶融合，在原生质体和再生水稻、小麦和玉米植物中，在原间隔区第 3 位到第 9 位实现了高达 43.48%的靶向胞嘧啶到胸腺嘧啶的转换频率。
