离子激流下一代测序技术揭示了锦鲤（鲤科）的完整线粒体基因组。
Ion Torrent next-generation sequencing reveals the complete mitochondrial genome of koi carp (, Cyprinidae).
作者信息
Li Xuezhu, Zeng Jie, Zhang Hetong, Weng Shaoping, He Jianguo, Dong Chuanfu
机构信息
MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, China.
出版信息
Mitochondrial DNA B Resour. 2016 Feb 5;1(1):34-36. doi: 10.1080/23802359.2015.1137807.
The complete mitochondrial genome of the koi carp (, Cyprinidae) was sequenced in the present study by using Ion Torrent Personal Genome Machine (PGM) platform for the first time. The mitochondrial genome sequence is 16 581 bp in size and consists of 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one control region. The gene order and organization were similar to most of the other teleost. The nucleotide compositions of the light strand are 24.82% of A, 31.92% of T, 27.53% of G and 15.73% of C. With the exception of eight tRNA genes and the NADH dehydrogenase subunit 6 (ND6), all other mitochondrial genes are encoded on the heavy strand. The phylogenetic tree constructed using a maximum-likelihood model showed sister relationship of koi carp to other Cyprinidae fishes.
本研究首次使用离子激流个人基因组测序仪（PGM）平台对锦鲤（鲤科）的完整线粒体基因组进行了测序。线粒体基因组序列大小为16581bp，由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区组成。基因顺序和组织与大多数其他硬骨鱼相似。轻链的核苷酸组成为：A占24.82%，T占31.92%，G占27.53%，C占15.73%。除8个tRNA基因和烟酰胺腺嘌呤二核苷酸脱氢酶亚基6（ND6）外，所有其他线粒体基因均由重链编码。使用最大似然模型构建的系统发育树显示锦鲤与其他鲤科鱼类为姐妹关系。
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