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使用Illunima HiSeq 2500对(雀形目:伯劳科)的线粒体全基因组进行测序。

Sequencing complete mitochondrial genome of (Passeriformes: Laniidae) using Illunima HiSeq 2500.

作者信息

Yang Mei-Xia, Wang Qing-Xiong, Xiao Hong, Yang Chao

机构信息

Shaanxi Institute of Zoology, Xi'an, China.

School of Life Sciences, Shaanxi Normal University, Xi'an, China.

出版信息

Mitochondrial DNA B Resour. 2016 Apr 19;1(1):306-307. doi: 10.1080/23802359.2016.1167640.

DOI:10.1080/23802359.2016.1167640
PMID:33473475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7799910/
Abstract

Using an Illumina platform, we sequenced the complete mitochondrial genome of to an average coverage of 1669.5×. We performed a assembly using SOAPdenovo2 and obtained the total mitogenome with 16,833 bp in length. Most PCGs begin with the typical ATG start codon with the exception of gene, which use GTG as the initiation codon. Stop codons AGG, TAG, TAA, and AGA are present in the PCGs; exceptions are and , which possess incomplete termination codons (T). But, the function of with incomplete stop codon T should be further investigated. The phylogeny revealed that genetic distance of Laniidae and Corvidae was closer than other species. Compared to other three shrike species, occupy a separate status in the genus

摘要

使用Illumina平台,我们对[物种名称]的完整线粒体基因组进行了测序,平均覆盖度为1669.5倍。我们使用SOAPdenovo2进行了组装,获得了长度为16,833 bp的完整线粒体基因组。大多数编码基因(PCGs)以典型的ATG起始密码子开始,但[基因名称]基因除外,它使用GTG作为起始密码子。编码基因中存在终止密码子AGG、TAG、TAA和AGA;例外的是[基因名称1]和[基因名称2],它们具有不完整的终止密码子(T)。但是,具有不完整终止密码子T的[基因名称]的功能应进一步研究。系统发育分析表明,伯劳科和鸦科的遗传距离比其他物种更近。与其他三种伯劳物种相比,[物种名称]在该属中占据独立的地位

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50bc/7799910/07526ac4cd60/TMDN_A_1167640_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50bc/7799910/07526ac4cd60/TMDN_A_1167640_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50bc/7799910/07526ac4cd60/TMDN_A_1167640_F0001_B.jpg

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本文引用的文献

1
Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads--a baiting and iterative mapping approach.直接从基因组下一代测序读段重建线粒体基因组——一种诱饵和迭代映射方法。
Nucleic Acids Res. 2013 Jul;41(13):e129. doi: 10.1093/nar/gkt371. Epub 2013 May 9.
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MITOS: improved de novo metazoan mitochondrial genome annotation.MITOS:改进的从头后生动物线粒体基因组注释。
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MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space.
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Mitochondrial DNA points to Lanius meridionalis as a polyphyletic species.线粒体DNA表明,南灰伯劳是一个多系物种。
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