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通过离子激流PGM二代测序技术对1912年的Ris(蜻蜓目:色蟌科)线粒体基因组进行研究。

The mitochondrial genome of Ris, 1912 (Zygoptera: Calopterygidae) via Ion Torrent PGM NGS sequencing.

作者信息

Xu Shaolin, Guan Zhaoying, Huang Qi, Xu Lei, Vierstraete Andy, Dumont Henri J, Lin Qiuqi

机构信息

Institute of Hydrobiology, Jinan University, Guangzhou, China.

School of Applied Biology, Shenzhen Institute of Technology, Shenzhen, China.

出版信息

Mitochondrial DNA B Resour. 2018 Jan 11;3(1):115-117. doi: 10.1080/23802359.2017.1413307.

DOI:10.1080/23802359.2017.1413307
PMID:33474087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7800031/
Abstract

The mitochondrial genome of was sequenced and assembled via Next-Generation Sequencing (NGS) and iteratively assembly process with a reference seed. This genome is 15,562 bp long and A + T biased (71%), with 37 genes arranged in common order of Odonata. All protein-coding genes are initiated by typical "ATN" codon, and 9 genes are terminated with a complete stop codon, except nad4, nad5, cox2, and cox3, which are terminated with an incomplete codon "T(aa)". The S5 intergenic spacer is absent in this genome, supporting that lacking of S5 as a specific character for damselflies. The A + T rich region of is 267 bp longer than that of . This mitogenome provides new molecular information for understanding of and .

摘要

通过下一代测序(NGS)和基于参考种子的迭代组装过程,对[物种名称]的线粒体基因组进行了测序和组装。该基因组长度为15,562 bp,具有A+T偏向性(71%),37个基因按蜻蜓目常见顺序排列。所有蛋白质编码基因均由典型的“ATN”密码子起始,9个基因以完整的终止密码子终止,除了nad4、nad5、cox2和cox3,它们以不完整密码子“T(aa)”终止。该基因组中不存在S5基因间隔区,支持缺乏S5作为豆娘的一个特定特征。[物种名称]的富含A+T区域比[另一物种名称]的长267 bp。这个线粒体基因组为理解[物种名称]和[另一物种名称]提供了新的分子信息。

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