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短读长测序组装揭示了1820年范德·林登(蜻蜓目:均翅亚目)的完整线粒体基因组。

Short read sequencing assembly revealed the complete mitochondrial genome of Vander Linden, 1820 (Odonata: Zygoptera).

作者信息

Feindt Wiebke, Herzog Rebecca, Osigus Hans-Jürgen, Schierwater Bernd, Hadrys Heike

机构信息

ITZ - Forschungsstätte 'Alter Bahnhof Schapen' Braunschweig, University of Veterinary Medicine Hannover, Hannover/Braunschweig, Germany.

Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, USA.

出版信息

Mitochondrial DNA B Resour. 2016 Aug 21;1(1):574-576. doi: 10.1080/23802359.2016.1192510.

DOI:10.1080/23802359.2016.1192510
PMID:33473559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7800176/
Abstract

Damselflies of the genus emerge as organisms with high potential in ecological, evolutionary and developmental research at the base of flying insects. and are for example one of the few odonate species where a complete life cycle over generations can be reared under laboratory conditions. We here report the complete mitochondrial genome of as a valuable genomic resource for future eco-evo-devo studies at the base of flying insects. The genome has a total length of 15,962 bp and displays all typical features of Odonata (dragonflies and damselflies) mitochondrial genomes in gene content and order as well as A + T content. Start and stop codons of all protein-coding genes are consistent. Most interestingly, we found four intergenic spacer regions and a long A + T rich (control) region of 1196 bp, which is almost double the size of the close relative . We assume that the adequate insert size and iterative mapping may be more efficient in assembling this duplicated and repetitive region.

摘要

该属豆娘作为飞行昆虫基部生态、进化和发育研究中具有高潜力的生物出现。例如,[具体物种A]和[具体物种B]是少数能在实验室条件下完成多代完整生命周期的蜻蜓目物种之一。我们在此报告[具体物种]的完整线粒体基因组,作为未来飞行昆虫基部生态 - 进化 - 发育研究的宝贵基因组资源。该基因组全长15962 bp,在基因内容、顺序以及A + T含量方面展现出蜻蜓目(蜻蜓和豆娘)线粒体基因组的所有典型特征。所有蛋白质编码基因的起始和终止密码子一致。最有趣的是,我们发现了四个基因间隔区和一个1196 bp的长A + T丰富(控制)区,其大小几乎是近缘种[具体物种C]的两倍。我们推测,合适的插入片段大小和迭代映射在组装这个重复区域时可能更有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fbd/7800176/aafa79b5775e/TMDN_A_1192510_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fbd/7800176/aafa79b5775e/TMDN_A_1192510_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fbd/7800176/aafa79b5775e/TMDN_A_1192510_F0001_B.jpg

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