Jiang Bin, Yao Yu, Li Jia, Zhang Jiang, Sun Yang, He Shulin
Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, China.
College of Life Sciences and Food Engineering, Shaanxi Xueqian Normal University, Xi'an, China.
Mitochondrial DNA B Resour. 2024 Aug 16;9(8):1081-1092. doi: 10.1080/23802359.2024.2389920. eCollection 2024.
Mitogenome data of Odonata is accumulating and widely used in phylogenetic analysis. However, noncoding regions, especially control region, were usually omitted from the phylogenetic reconstruction. In an effort to uncover the phylogenetic insights offered by the control region, we have amassed 65 Odonata mitogenomes and conducted an examination of their control regions. Our analysis discovered that species belonging to Anisoptera and Anisozygoptera exhibited a stem-loop structure, which was formed by a conserved polyC-polyG stretch located near the gene (encoding 12S rRNA). Conversely, the polyC-polyG region was not a conserved fragment in Zygoptera. The length and number of repetitions within the control region were identified as the primary determinants of its overall length. Further, sibling species within Odonata, particularly those in the genus , displayed similar patterns of repetition in their control region. Collectively, our research delineates the structural variations within the control region of Odonata and suggests the potential utility of this region in elucidating phylogenetic relationships among closely related species.
蜻蜓目线粒体基因组数据不断积累，并广泛应用于系统发育分析。然而，在系统发育重建中，非编码区，尤其是控制区，通常被忽略。为了揭示控制区提供的系统发育见解，我们收集了65个蜻蜓目线粒体基因组，并对其控制区进行了研究。我们的分析发现，差翅亚目和间翅亚目的物种呈现出一种茎环结构，该结构由位于基因（编码12S rRNA）附近的保守聚C-聚G序列形成。相反，聚C-聚G区域在均翅亚目中不是一个保守片段。控制区内重复序列的长度和数量被确定为其总长度的主要决定因素。此外，蜻蜓目内的亲缘种，特别是属内的那些物种，在其控制区表现出相似的重复模式。总体而言，我们的研究描绘了蜻蜓目控制区的结构变异，并表明该区域在阐明近缘物种间系统发育关系方面的潜在用途。
