Kumar Ved P, Shukla Malay, Rajpoot Ankita, Thakur Mukesh, Nigam Parag, Kumar Dhyanendra, Mehta Anil Kumar, Goyal Surendra P
Wildlife Forensic Cell, Wildlife Institute of India, Dehradun, Uttarakhand, India.
Department of Zoology, Veer Kunwar Singh University, Arrah, Bihar, India.
Mitochondrial DNA B Resour. 2018 Feb 20;3(1):250-255. doi: 10.1080/23802359.2018.1438858.
DNA barcoding has become a popular method of choice for identification of specimen based on molecular techniques. Here, we present preliminary findings on generating robust DNA barcode library of Cervids of India. The dataset comprising the DNA barcode library of seven deer species included in the genus and classified under family Cervidae. Mitochondrial Cytochrome C Oxidase subunit I gene of ca. 710 bp accepted widely as DNA barcode region, was used for generating species specific signature from 31 known samples of seven Indian deer species. Expectedly, the NJ tree clustered three genera i.e. and of Cervids of India into three clades. Further, the intra- and interspecies distances based on Kimura 2 parameter model also supported the results. The average intra- and interspecies sequence divergence were 0.011 (±0.09) and 0.65 (±0.14), respectively. The present study has exhibited that DNA barcoding has discriminating power to delineate boundaries among the closely related species. The data generated are of high importance to the law enforcement agencies in effective identification of species in wildlife offence cases. The similar approach can be utilized for generating DNA barcodes for other Indian mammals for making effective management and conservation action decisions.
DNA条形码已成为基于分子技术鉴定标本的一种常用方法。在此，我们展示了关于构建印度鹿科动物强大DNA条形码文库的初步研究结果。该数据集包含鹿科中七个鹿种的DNA条形码文库，这些鹿种被归类于鹿科。约710 bp的线粒体细胞色素C氧化酶亚基I基因被广泛认可为DNA条形码区域，用于从七个印度鹿种的31个已知样本中生成物种特异性特征。不出所料，NJ树将印度鹿科的三个属，即 和 聚为三个分支。此外，基于Kimura 2参数模型的种内和种间距离也支持了这些结果。种内和种间序列平均分歧分别为0.011（±0.09）和0.65（±0.14）。本研究表明，DNA条形码具有区分能力，可界定近缘物种之间的界限。所生成的数据对于执法机构在野生动物犯罪案件中有效识别物种具有高度重要性。类似的方法可用于为其他印度哺乳动物生成DNA条形码，以做出有效的管理和保护行动决策。
