Wang Ah Rha, Kim Min Jee, Jeong Su Yeon, Kim Iksoo
Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Mitochondrial DNA B Resour. 2018 Feb 24;3(1):282-283. doi: 10.1080/23802359.2018.1443040.
The tiger beetle, Chevrolat, 1845 (Coleoptera: Carabidae), has been listed as an Endangered insect in South Korea. We sequenced the complete mitochondrial genome (mitogenome) of this organism (16,388 bp). The genome includes a typical set of genes (13 protein-coding genes (PCGs), 2 rRNA genes, and 22 tRNA genes) and 1 non-coding region with an arrangement identical to that observed in most insect genomes. Twelve PCGs had the typical ATN start codon, whereas had the atypical TTG codon. The AT-rich region is 1629-bp long, composed of 80.0% A + T nucleotides, and has no long repeat sequences. Phylogenetic analyses with concatenated sequences of the 13 PCGs and 2 rRNA genes, using the Bayesian inference (BI) method, placed as a sister to the within-subfamilial species , with the highest nodal support presented by both BI and maximum likelihood (ML) methods. Three subfamilies represented by more than one species (Cicindelinae, Harpalinae, and Carabinae) were all determined by both BI and ML analyses to form strong monophyletic groups.
1845年的虎甲（鞘翅目：步甲科）已被列为韩国的濒危昆虫。我们对该生物的完整线粒体基因组（线粒体基因组）进行了测序（16,388 bp）。该基因组包括一组典型的基因（13个蛋白质编码基因（PCG）、2个rRNA基因和22个tRNA基因）以及1个非编码区，其排列与大多数昆虫基因组中观察到的排列相同。12个PCG具有典型的ATN起始密码子，而 具有非典型的TTG密码子。富含AT的区域长1629 bp，由80.0%的A + T核苷酸组成，并且没有长重复序列。使用贝叶斯推断（BI）方法对13个PCG和2个rRNA基因的串联序列进行系统发育分析，将 置于亚科内物种 的姐妹位置，BI和最大似然（ML）方法均给出了最高的节点支持。由多个物种代表的三个亚科（虎甲亚科、婪步甲亚科和步甲亚科）均通过BI和ML分析确定形成强大的单系类群。
