Department of Plastic, Reconstructive and Maxillo facial Surgery, Henri Mondor Hospital, University Paris XII, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France.
Department of Plastic and Reconstructive Surgery, Università Campus Biomedico di Roma, Via Alvaro del Portillo, 21, 00128, Roma, Italy.
Aesthetic Plast Surg. 2021 Jun;45(3):1282-1293. doi: 10.1007/s00266-020-02100-7. Epub 2021 Jan 20.
Striae distensae (SD) appear clinically as parallel striae, lying perpendicular to the tension lines of the skin. SD evolve into two clinical phases, an initial inflammatory phase in which they are called "striae rubrae" (SR) and a chronic phase in which they are called striae albae (SA). Fibroblasts seem to play a key role in the pathogenesis of stretch marks. This study was aimed at describing and analyzing stretch marks-derived fibroblasts (SMF), the differences between SR- and SA-derived fibroblasts (SRF, SAF), testing two treatments in vitro (sodium ascorbate and PrP) on SAF.
To characterize the SMF, the expression of alpha smooth muscle actin (alpha SMA) was investigated. Type I collagen expression was measured in SAF, before and after adding different PrP concentrations and sodium ascorbate in the culture medium. Results were processed through statistical analysis models using the Student's t-test.
A significant increase in alpha SMA (P <0.001) was observed in SRF. SAF treated with PrP and sodium ascorbate showed a resumption of their metabolic activity by an increase in collagen type I production and cell proliferation. After 24 h of incubation with PrP 1% and PrP 5% + sodium ascorbate, cell viability was increased by 140% and 151% and by 156 and 178% after 48 h, respectively, compared to the control.
Our study shows that a biologically mediated improvement in SMF metabolic activity is possible. Our promising results require further trials to be able to confirm the reproducibility of this combined treatment, particularly in vivo.
This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable.
妊娠纹（SD）临床上表现为平行的条纹，与皮肤的张力线垂直。SD 分为两个临床阶段，初始炎症期称为“红色妊娠纹”（SR），慢性期称为白色妊娠纹（SA）。成纤维细胞似乎在妊娠纹的发病机制中起关键作用。本研究旨在描述和分析来自妊娠纹的成纤维细胞（SMF），比较 SR 期和 SA 期成纤维细胞（SRF、SAF）之间的差异，在体外（抗坏血酸钠和 PrP）测试两种治疗方法对 SAF 的影响。
为了表征 SMF，研究了α平滑肌肌动蛋白（α SMA）的表达。在 SAF 中测量了 I 型胶原蛋白的表达，分别在添加不同浓度 PrP 和抗坏血酸钠前后测量。结果通过使用 Student's t 检验的统计分析模型进行处理。
SRF 中α SMA 的表达显著增加（P <0.001）。用 PrP 和抗坏血酸钠处理的 SAF 表现出通过增加 I 型胶原蛋白的产生和细胞增殖来恢复其代谢活性。与对照组相比，孵育 1% PrP 和 5% PrP+抗坏血酸钠 24 h 后，细胞活力分别增加了 140%和 151%，孵育 48 h 后分别增加了 156%和 178%。
我们的研究表明，SMF 代谢活性的生物介导改善是可能的。我们有前途的结果需要进一步的试验来确认这种联合治疗的重现性，特别是在体内。
本期刊要求作者为每个提交的内容分配一个证据级别，以便对循证医学排名进行应用。
