Shi Rui, Zhang Jingshuang, Niu Kun, Li Weiyang, Jiang Ni, Li Jianlin, Yu Qingsong, Wu Chengai
Beijing Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing 100035, China.
Biomater Sci. 2021 Mar 21;9(6):2090-2102. doi: 10.1039/d0bm01304h. Epub 2021 Jan 21.
Deferoxamine (DFO), an iron chelator regarded as a hypoxic analogue, has been reported to be involved in angiogenesis and osteogenic differentiation. In this study, DFO was loaded into nanospheres, Then, DFO-loaded NPs and free DFO were co-encapsulated in nanofibers through coaxial electrospinning and its effects on cell viability, migration, and osteogenic differentiation, and the potential mechanisms were investigated. The results suggested that DFO maintained cell viability and promoted the migration of human mesenchymal stem cells (hMSCs) and MC3T3-E1 cells. ALP activity, calcium deposition, and expression of osteogenesis-related markers, including collagen, osteocalcin, and osteopontin, were all increased with DFO. Moreover, hypoxia-inducible factor-1α, transforming growth factor-β, and Smad2 were upregulated with DFO, which indicated activation of the TGF-β1/Smad2 signalling pathway. This may contribute to osteogenic differentiation of cells.
去铁胺（DFO）是一种被视为缺氧类似物的铁螯合剂，据报道其参与血管生成和成骨分化。在本研究中，将DFO负载到纳米球中。然后，通过同轴静电纺丝将负载DFO的纳米颗粒和游离DFO共包封在纳米纤维中，并研究其对细胞活力、迁移和成骨分化的影响以及潜在机制。结果表明，DFO维持细胞活力并促进人间充质干细胞（hMSCs）和MC3T3-E1细胞的迁移。DFO处理后，碱性磷酸酶活性、钙沉积以及包括胶原蛋白、骨钙素和骨桥蛋白在内的成骨相关标志物的表达均增加。此外，DFO上调缺氧诱导因子-1α、转化生长因子-β和Smad2，这表明TGF-β1/Smad2信号通路被激活。这可能有助于细胞的成骨分化。
